Pharmaceutical
Communication
Biosci. Biotech. Res. Comm. 10(1): 44-50 (2017)
Evaluation of antimicrobial and synergistic effects of
selected medicinal plants of Hail area with antibiotics
Abdel Moneim E. Sulieman, Sherif M. Shaarawy, Ahmed A. Alghamdi, Vajid N. Veettil*,
Mohanad Abdelgadir and Nasir A. Ibrahim
Department of Biology, Faculty of Science, University of Hail, Hail, Kingdom of Saudi Arabia
ABSTRACT
In this study, the antibacterial activity of selected medicinal plants and their synergistic effect with antibiotics were
investigated. Different antibiotic resistant bacterial species were employed including: Escherichia coli (E. coli), Kleb-
siella pneumoniae (K. pneumonia), Acinetobacter baumannii (A.baumannii), Pseudomonas aeruginosa (P. aeruginosa),
Staphylococcus aureus (S. aureus) and Salmonella spp. The results indicated that methanol extracts of most of the
tested plants inhibited growth of the tested bacteria, however, the highest inhibition was by Senna followed by Har-
mal, saffower and Guaada, where the inhibition zone diameter was 8 mm, 4 mm, 3 mm, 2 mm and 2 mm, respec-
tively. The synergism between plants extract and antibiotics resulted in inhibition of the tested organisms; generally,
the synergism with Harmal extract was more effective in inhibition of antibiotic-resistant bacteria, followed by Senna
and fnally Saffower. The outcomes additionally demonstrated that methanolic concentrate of the plant has more
inhibitory impact than that of the water extract. Further investigation of the plants extract to seclude and recognize
the dynamic fxings is prescribed.
KEY WORDS: ANTIBIOTIC-RESISTANT BACTERIA, INHIBITION ZONE, SAFFLOWER, METHANOL.
44
ARTICLE INFORMATION:
*Corresponding Author: vajidnv@gmail.com
Received 12
th
Jan, 2017
Accepted after revision 22
nd
March, 2017
BBRC Print ISSN: 0974-6455
Online ISSN: 2321-4007 CODEN: USA BBRCBA
Thomson Reuters ISI ESC and Crossref Indexed Journal
NAAS Journal Score 2017: 4.31 Cosmos IF : 4.006
© A Society of Science and Nature Publication, 2017. All rights
reserved.
Online Contents Available at: http//www.bbrc.in/
INTRODUCTION
Plants as a source of medicinal compounds have kept on
assuming to play a dominant role in the maintenance
of human health since ancient times. As indicated by
the World Health Organization plant extracts or their
dynamic constituents are utilized as people prescription
in customary treatments of 80% of the total population.
More than half of all modern clinical drugs are of regu-
lar item source (Kirbag et al., 2009). Medicinal plants
possess immunomodulatory and antioxidant properties,
prompting to antibacterial activities. They are known
to have adaptable immunomodulatory activity by
stimulating both non-particular and particular immu-
nity (Pandey and Chowdhry, 2006). In the most recent
couple of years, various reviews have been directed in