Trad. Med. J., May-August 2022 Vol. 27(2), p 146-152 ISSN-p : 1410-5918 ISSN-e : 2406-9086 146 DOI: 10.22146/mot.74667 | Traditional Medicine Journal, 27(2), 2022 Submitted : 16-05-2022 Revised : 01-07-2022 Accepted : 15-08-2022 Standardization of Simplicia Golden Sea Cucumber (Stichopus hermanii) from Pelapis Island, West Kalimantan Wintari Taurina * , Mohamad Andrie Department of Pharmacy, Faculty of Medicine, Tanjungpura University, Pontianak, Indonesia ABSTRACT Standardization of medicinal ingredients and drug preparations is a requirement to ensure reproducibility of pharmaceutical and therapeutic quality. With standardization, it is hoped that it can further increase trust in medicines derived from natural ingredients. Golden sea cucumber is one of the natural ingredients that can be used as raw material for wound healing ointment preparations. The main content in golden sea cucumbers that play a role in wound healing is protein. This study aims to standardize the Simplicia of golden sea cucumber (Stichopus hermanii) originating from Pelapis Island, West Kalimantan. Standardization uses physical, chemical, and microbiological parameters like raw material requirements for standardized herbal medicines (OHT). The test is carried out in the laboratory using the method according to SNI. The results of the Simplicia test of golden sea cucumbers from Pelapis Island, West Kalimantan, included water content of 9.57%, zinc 9.32 mg/kg, cadmium < 0.030 mg/kg, copper < 0.030 mg/kg, lead < 0.066 mg/kg , mercury < 0.007 mg/kg, Total Plate Number 8.0 x 103 colonies/g, E.Coli < 3 APM/gr and Salmonella negative have fulfilled the requirements. The results of the Simplicia test of golden sea cucumbers include 13.4% ash content, 1.52% acid insoluble ash, 36.1% protein, 1.39% total fat, 13.1 mg/kg magnesium, and 10.6 mg iron. /kg. The results of the phytochemical screening of golden sea cucumber Simplicia were positive for containing alkaloids, saponins, and triterpenoids. Keywords : standardization; Simplicia; golden sea cucumber; Stichopus hermanii; wound healing ointment INTRODUCTION Standardization is an effort to improve product quality and safety. Standardization of drug ingredients and drug preparations is a requirement to ensure the reproducibility of pharmaceutical and therapeutic quality. With standardization, it is hoped that it can further increase trust in medicines derived from natural ingredients. Standardization of medicinal materials includes starting materials, intermediates, or finished products. One of the medicinal ingredients that are often used in natural medicinal preparations is Simplicia. Standardization of Simplicia needs to be done to get a repeatable effect (reproducible) (Wijaya, Setiawan, and Purnama, 2019). The golden sea cucumber (Stichopus hermanii) is one of the marine animals classified as marine invertebrates belonging to the phylum Echinoderms (Pringgenies, Rudiyanti, and Yudiati, 2018). Sea cucumbers can be found in all coastal waters from shallow tidal areas to deeper waters. The main habitats of sea cucumbers are coral and seagrass beds. The distribution of sea cucumbers in Indonesia, among others, in East Java, Madura, Sumba, Bali, Aceh, Lombok, Bangka, Bengkulu, *Corresponding author : Wintari Taurina Email : wintari.taurina@pharm.untan.ac.id Riau and surrounding areas, Belitung, Maluku, Papua, Sulawesi, Thousand Islands, and Kalimantan (west, east and south) (Leha et al., 2020). Sea cucumbers contain protein and this protein has many benefits for the body. According to Rasyid (2018), sea cucumbers contain 86% protein that is easily soluble in the pepsin enzyme (Rasyid, 2018). Sea cucumbers, apart from being a source of protein, are also known to be used as medicine. Sea cucumbers have properties like anti- inflammatory, antioxidant, antiviral, antimicrobial, preventing blood clots/clotting, accelerating wound healing, preventing arthritis, high protein food source, anti-fatigue, boosting the immune system, and preventing cancer (Nurwidodo et al., 2018). Sea cucumbers can regenerate themselves within 10-90 days so that they are whole again because sea cucumbers contain cell growth factors and high protein content. The ability of sea cucumbers to regenerate cells is the main basis that sea cucumbers can heal wounds. Therefore, sea cucumbers can be used as an ointment to heal wounds (Suryaningrum, 2008). Simplicia of golden sea cucumber is a natural ingredient that can be used as a raw material for wound healing ointments. This study aims to standardize the Simplicia of golden sea cucumber (Stichopus hermanii) originating from Pelapis Island, West Kalimantan. Standardization