FEMS Microbiology Ecology, 96, 2020, faa024 doi: 10.1093/femsec/faa024 Advance Access Publication Date: 13 February 2020 Research Article RESEARCH ARTICLE Tannin supplementation modulates the composition and function of ruminal microbiome in lambs infected with gastrointestinal nematodes Patricia Spoto Corr ˆ ea 1,2,6, *, Lucas William Mendes 2 , Leandro Nascimento Lemos 2 , Pierre Crouzoulon 1 , Vincent Niderkorn 3 , Herv ´ e Hoste 4 , Livio Martins Costa-J ´ unior 5 , Siu Mui Tsai 2 , Antonio P. Faciola 6 , Adibe Luiz Abdalla 1 and Helder Louvandini 1,6 1 Laboratory of Animal Nutrition, Center for Nuclear Energy in Agriculture, University of S˜ ao Paulo, 13400–970, Piracicaba, SP, Brazil, 2 Laboratory of Molecular Cell Biology, Center for Nuclear Energy in Agriculture, University of S ˜ ao Paulo, 13400–970, Piracicaba, SP, Brazil, 3 Universit ´ e Clermont Auvergne, INRAE, VetAgro Sup, UMR Herbivores, F-63122 Saint-Gen ` es Champanelle, France, 4 INRAE, UMR, Chemin des Capelles – BP 87614 – 31 076 – Toulouse, France, 5 Biological and Health Center, Federal University of Maranhao, Avenida dos Portugueses, 1966 - Vila Bacanga, 65080-805, S˜ ao Lu´ ıs - Maranhao, Brazil and 6 Department of Animal Sciences, University of Florida, Gainesville 32611, United States ∗ Corresponding author: Universidade de Sao Paulo Centro de Energia Nuclear na Agricultura Av. Centenario, 303, CP 96 Piracicaba, SP 13400-970 Brazil. Tel: +55-19-3429-4785; E-mail: patricia.louvandini@gmail.com One sentence summary: The metagenome and ruminal parameters was used to understand the effect of tannin supplementation on rumen microbiota of lambs infected with Haemonchus contortus and Trichostrongylus colubriformis. Editor: Leluo Guan ABSTRACT This study was carried out to evaluate the effects of tannin supplementation on ruminal microbiota of sixteen lambs infected and non-infected with Haemonchus contortus and Trichostrongylus colubriformis. Animals were fed with hay, concentrate and supplemented with Acacia mearnsii (A. mearnsii). The animals were divided into four treatments: two control groups without infection, either receiving A. mearnsii (C+) or not (C-), and two infected groups, one with A. mearnsii (I+) and another without A. mearnsii (I-). Ruminal short-chain fatty acids (SCFA) and metagenome sequencing of ruminal microbiota were used to evaluate the effect of tannin and infection on ruminal microbiome. For SCFA, differences were observed only with A. mearnsii. Total SCFA and acetate molar percentage were decreased in C+ and I+ (P<0.05). Butyrate, valerate and isovalerate were higher in lambs that received A. mearnsii in the diet (P<0.05). The infection changed the microbiome structure and decreased the abundance of butyrate-producing microorganisms. In addition, A. mearnsii supplementation also affected the structure the microbial community, increasing the diversity and abundance of the butyrate-producing and probiotics bacteria, amino acid metabolic pathways, purine, pyrimidine and sphingolipid metabolism. Together, our fndings indicate that A. mearnsii supplementation modulates important groups related to nitrogen, amino acid, purine and pyrimidine metabolism, in rumen microbiome, affected by gastrointestinal nematodes infection in lambs. Received: 16 August 2019; Accepted: 12 February 2020 C  FEMS 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 1 Downloaded from https://academic.oup.com/femsec/article/96/3/fiaa024/5735434 by guest on 02 February 2024