International Journal of Pharmaceutical Research and Applications Volume 6, Issue 4 July-Aug 2021, pp: 658-662 www.ijprajournal.com ISSN: 2249-7781 DOI: 10.35629/7781-0604658662 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 658 “A Review On Recombinant Dna Technology: Importance And Progress” Ali Salman Al-Shami, 1 * Deepak Kumar Jha 2 1, 2, Department of Pharmacology, Karnataka College of Pharmacy, Karnataka, India --------------------------------------------------------------------------------------------------------------------------------------- Date of Submission: 15-07-2021 Date of Acceptance: 31-07-2021 --------------------------------------------------------------------------------------------------------------------------------------- ABSTRACT Assuredly, the recombinant DNA technology has emerged to improve the quality of human life. In this paper, definition, procedures, types and applications of recombinant DNA technology have been described. There are three different methods of recombinant DNA technology; transformation, non-bacterial transformation and phage introduction. The recombinant DNA technology has several applications in various fields such as agriculture, public health, gene therapy, environmental science and pollution research, clinical pharmacy and development of hormones and vaccines. Keywords: Recombinant DNA, Biotechnology, Genetic engineering. I. INTRODUCTION Improving the quality of human life is the ultimate goal for every nation. Generally, the human life is influenced by three main priorities; scarcity of the food, health issues and environmental complications (Khan et al., 2016). Numerous human health related problems are considered the most critical factor associated with deterioration of human life quality. Several diseases such as HIV, malaria, T.B. and dengue are threatening human life based on the global statistics from (Khan et al., 2016). Structure and function of DNA Dioxyribonucleic Acia (DNA) is a molecule located in the nucleus of the cells and is the storage of the genetic information about an organism. It is responsible for control the development and function of the living organism. The DNA transfer the genetic information through the generations. Chemically, the DNA consists of three main components; phosphate group, sugar (ribose lack oxygen) and nucleotide base of purine or pyrimidine as pairs adenine (A) paired with thymine (T), and guanine (G) paired with cytosine (C) (Hayward, 1991). The bonding of these three components produced a “double helix” structure as shown in Figure 1. This structure was discovered by James Watson and Francis Crick on 1953. The sequence of nucleotides (C,T,G,A) comprises what we call it “gene” which is translated intro a specific protein through a process of protein synthesis with involvement of ribonucleic acid (RNA) and ribosomes. Figure 1: Structure of the deoxyribonucleic acid (DNA). Adapted from the National Cancer Institute (NIH) at https://www.cancer.gov/publications/dictionaries/genetics-dictionary/def/dna