Research Article Impact of Atmospheric Microparticles on the Development of Oxidative Stress in Healthy City/Industrial Seaport Residents Kirill Golokhvast, 1,2 Tatyana Vitkina, 1 Tatyana Gvozdenko, 1 Victor Kolosov, 3 Vera Yankova, 1 Elena Kondratieva, 1 Anna Gorkavaya, 1 Anna Nazarenko, 1 Vladimir Chaika, 2 Tatyana Romanova, 4 Alexander Karabtsov, 4 Juliy Perelman, 3 Pavel Kiku, 2 and Aristidis Tsatsakis 5 1 Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia 2 Far Eastern Federal University, 8 Sukhanova Street, Vladivostok 690950, Russia 3 Far Eastern Center of Physiology and Pathology of Respiration, 22 Kalinina Street, Blagoveshchensk 675000, Russia 4 Far Eastern Geological Institute FEB RAS, 159 Prospekt 100-letiya, Vladivostok 690022, Russia 5 Department of Toxicology and Forensics, Medical School, University of Crete, Heraklion, 71300 Crete, Greece Correspondence should be addressed to Kirill Golokhvast; droopy@mail.ru Received 4 November 2014; Revised 7 April 2015; Accepted 8 April 2015 Academic Editor: Vladimir Jakovljevic Copyright © 2015 Kirill Golokhvast et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Atmospheric microsized particles producing reactive oxygen species can pose a serious health risk for city residents. We studied the responses of organisms to microparticles in 255 healthy volunteers living in areas with diferent levels of microparticle air pollution. We analyzed the distribution of microparticles in snow samples by size and content. ELISA and fow cytometry methods were employed to determine the parameters of the thiol-disulfde metabolism, peroxidation and antioxidant, genotoxicity, and energy state of the leukocytes. We found that, in the park areas, microparticles with a size of 800 m or more were predominant (96%), while in the industrial areas, they tended to be less than 50 m (93%), including size 200–300 nm (7%). In the industrial areas, we determined the oxidative modifcation of proteins (21% compared to the park areas,  ≤ 0.05) and DNA (12%,  ≤ 0.05), as well as changes in leukocytes’ energy potential (53%,  ≤ 0.05). An increase in total antioxidant activity (82%,  ≤ 0.01) and thiol-disulfde system response (thioredoxin increasing by 33%,  ≤ 0.01; glutathione, 30%,  ≤ 0.01 with stable reductases levels) maintains a balance of peroxidation-antioxidant processes, protecting cellular and subcellular structures from signifcant oxidative damage. 1. Introduction Te development of the research direction of city ecology was caused by the global challenges which humanity is facing due to the increasing industrial activity. Furthermore, the world urban population continues to grow and so does the prevalence of environmental diseases. City air is the frst marker of total environmental pollution. Atmospheric pollution in general is one of the leading factors related to health risks in cities [1–3]. Of all atmospheric particles, microsized particles are the most dangerous [4], especially those smaller than 10 m. Such particles slowly disappear from the atmosphere and have a relatively long life in a suspended state. Tey signifcantly accumulate in the envi- ronment even at a considerable distance from the sources of pollution. Unlike gases, microparticles are heterogeneous complex mixtures consisting of numerous components and their properties vary widely in terms of time and space [5]. It has been shown that long-term exposure to high levels of particulate matter <2.5 m in aerodynamic diameter (PM 2.5 ) in the atmosphere reduces life expectancy among the population from several months to a few years [6]. In addition, suspended particles absorb a large amount of toxic substances that can enter an organism [7]. Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2015, Article ID 412173, 10 pages http://dx.doi.org/10.1155/2015/412173