Vol.10 (2020) No. 6 ISSN: 2088-5334 Non-destructive Measurement of Lycopene Content in High Soluble Solids Stored Tomato (Solanum Lycopersicum Mill. cv Rinka 409) Fanesya Dyah Anggraeni a,1 , Nafis Khuriyati a,2 , Moh. Affan Fajar Falah a,3 , Hiroshige Nishina b,1 , Kotaro Takayama b,2 , Noriko Takahashi b,3 a Graduate School of Agroindustrial Technology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia Email: 1 fanesya.dyah.a@mail.ugm.ac.id; 2 nafis.khuriyati@ugm.ac.id; 3 affan_tip@ugm.ac.id b Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan Email: 1 nishina@ehime-u.ac.jp; 2 takayama@agr.ehime-u.ac.id; 3 takahashi.noriko.mm@ehime-u.ac.jp Abstract— High-quality fruits can be produced by controlling the environmental factors, i.e. temperature, relative humidity, carbon dioxide, and light intensity. The tomato (Solanum lycopersicum) variety ‘Rinka 409’, was grown hydroponically with a high-wire system in high technology greenhouse, Ehime University, Japan. The water stress treatment using visual monitoring system was used to produce the high soluble solids tomato fruits. Moreover, a high lycopene content of tomato can be produced by controlling the storage condition. Non-destructive measurement has been known to provide a fast and accurate quality measurement of fruits and vegetables. The objectives of this study were to develop a precise position for the non-destructive measurement of the lycopene content in high soluble solids stored tomato using Vis/NIR spectroscopy and evaluate its performance. In this study, the high soluble solids of tomato fruits were grown under water stress treatment and stored after harvesting at 25°C in a cool incubator for 7 days. The tomato spectra were measured using Vis/NIR spectroscopy with the wavelength 500-1010 nm. The estimation of the lycopene content was based on the statistical model using multivariate analysis. From this study, it is concluded that the measurement of lycopene content in high soluble solids of tomato fruits after storage by using the top part of the tomato, was better than if it is compared to the side part. It was best determined using PLS analysis with Visible/Near-infrared Spectroscopy non-destructively, with the R 0.98 and RMSE 0.80. Keywords— high soluble solids tomato; lycopene; non-destructive measurement; stored tomato; visible/near-infrared spectroscopy. I. INTRODUCTION In high technology greenhouse, the environmental factors can be controlled for optimal plant growth. Quality is a high priority for greenhouse crops, requiring much care in pest and disease management, not only to secure yields but also to obtain a high-value product. Greenhouse technology uses solar irradiance to support the production of plant and to protect the plant against external environment [1]. In the high technology greenhouse, the speaking plant has been studied for years, especially at Ehime University, Japan. The speaking plant is the idea of using observed crop processes as the variables to be controlled. The use of internet technology for environmental control in the greenhouse has been used for years [2] as an online monitoring of crop responses as early warning systems to detect and prevent the unintended condition by giving substantial resources needed [3]. Online monitoring system can be used to control the greenhouse environment, such as temperature, humidity, light intensity, and other environmental information using WiFi and it provides a stable, feasible, and low-cost solution for greenhouse production management [4]. The artificial lighting in a greenhouse can be used not only for replacing or supplementing the sunlight but also for boosting growth and is designed by the crop’s photoperiodicity, natural day length, average hours of sunlight, solar radiation angle and intensity, the amount of structure-induced shading. By using high technology, the high soluble solids of fruits can be produced in the greenhouse. The high soluble solids tomato fruits can be produced using water stress treatment with an early detection technique based on the projected plant area calculated from digital color images captured by digital camera [5]. The water stress is known can give positive effect to soluble solids content (SSC), with more than 10% increase compared to controls [6], [7]. Tomato (Solanum Lycopersicum Mill.) is one of the world’s most popular vegetables. Tomato can be grown in the home garden and commercially. Tomato is widely used 2567