Research Article Dietary Methanolic Extract of Fenugreek Enhanced the Growth, Haematobiochemical,ImmuneResponses,andResistanceagainst Aeromonas hydrophila in Nile Tilapia, Oreochromis niloticus AmanyM.Diab, 1 BasantT.Al-Khefa, 2 MalikM.Khalafallah, 2 AbdallahS.Salah, 2,3 FoadA.Farrag, 4 andMahmoudA.O.Dawood 5,6 1 Aquatic Microbiology, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafr El•Sheikh, Egypt 2 Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh 33516, Egypt 3 Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK 4 Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt 5 Animal Production Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El•Sheikh 33516, Egypt 6 Te Center for Applied Research on the Environment and Sustainability, Te American University in Cairo, Cairo 11835, Egypt Correspondence should be ddressed to Mhmoud A. O. Dwood; mhmoud•dwood@ucegypt.edu Received 19 September 2022; Revised 1 November 2022; Accepted 11 January 2023; Published 4 February 2023 Acdemic Editor: Hmed Ghfrifrsni Copyright © 2023 Amny M. Dib et l. Tis is n open ccess rticle distributed under the Cretive Commons Attribution License, which permits unrestricted use, distribution, nd reproduction in ny medium, provided the originl work is properly cited. Fenugreek seed (FS) contins bundnt functionl biomolecules tht ctivte the ntioxidtive nd immunity system of qutic nimls. In this study, the methnolic extrct of FS ws included in Nile tilpi diets t 0, 0.05, 0.1, 0.15, nd 0.2% levels nd fed to fsh for 90dys. Nile tilpi (10.57 ± 0.14g) were rndomly divided into fve triplicte groups to check the growth performnce, hemtobiochemicl profle, immunity, ntioxidtive response, nd tolernce to Aeromonas hydrophila infection. Te results reveled tht the methnolic extrct of FS signifcntly improved the growth performnce while reducing the feed conversion rtio (FCR). Methnolic extrct of FS lso modulted the hemtobiochemicl profle. Mrkedly, the lysozyme nd phgocytic ctivities were ctivted by the dietry methnolic extrct of FS. Te superoxide dismutse (SOD) ctivity ws improved, while the mlondildehyde (MDA) level ws decresed by the dietry methnolic extrct of FS. Te SOD ws mrkedly incresed in the fsh• feddietrymethnolicextrctofFSt0.1,0.15,nd0.2%,whiletheMDAdecresedinthe fsh fed 0.15 nd 0.2%. Te trnscription of IL•1β, TNF•α, nd TGF•β genes showed upregulted expression by ethnolic extrct of FS. Accordingly, Nile tilpi showed high resistnce ginst the A. hydrophila infection. Te regression nlysis reveled tht the inclusion of 0.09% is recommended to improve the specifc growth rte nd FCR. In conclusion, the study reveled tht n ethnolic extrct of FS is recommended to enhnce the growth performnce, immunity, ntioxidtive response, nd tolernce to A. hydrophila in Nile tilpi. 1.Introduction Nile tilpi (Oreochromis niloticus) is one of the most im• portnt economic fnfsh species worldwide, nd its pro• duction is incresing stedily [1, 2]. However, signifcnt economic losses hve been observed in the tilpi qu• culture sector in recent yers due to the spred of infectious diseses [3]. As  result of the spred of diseses in tilpi frms,  group of chemicl tretments were used on  lrge scle to prevent pthogens, such s ntibiotics [4, 5]. Te continued use of these chemicls led to the emergence of wht is known s dvnced ntibiotic resistnce nd the ccumultion of remnnts of these chemotherpies in the fsh body [6]. Hence, it is necessry to resort to n lterntive tretment, such s using nturl functionl substnces to limit the spred of infectious diseses nd control the pthogen [7]. Medicinl herbs nd their extrcts re used s n l• terntive to ntibiotic therpy s n environmentlly friendly component tht contributes to incresing Hindawi Aquaculture Research Volume 2023, Article ID 3055476, 13 pages https://doi.org/10.1155/2023/3055476