Journal Pre-proof Reverse and structural vaccinology approach to design a highly immunogenic multi-epitope subunit vaccine against Streptococcus pneumoniae infection Lohany Dias Mamede, Keila Gonçalves de Paula, Bianca de Oliveira, Janete Soares Coelho dos Santos, Lucas Maciel Cunha, Moacyr Comar Junior, Lenice Roteia Cardoso Jung, Alex Gutterres Taranto, Débora de Oliveira Lopes, Sophie Yvette Leclercq PII: S1567-1348(20)30304-X DOI: https://doi.org/10.1016/j.meegid.2020.104473 Reference: MEEGID 104473 To appear in: Infection, Genetics and Evolution Received date: 31 March 2020 Revised date: 16 July 2020 Accepted date: 19 July 2020 Please cite this article as: L.D. Mamede, K.G. de Paula, B. de Oliveira, et al., Reverse and structural vaccinology approach to design a highly immunogenic multi-epitope subunit vaccine against Streptococcus pneumoniae infection, Infection, Genetics and Evolution (2020), https://doi.org/10.1016/j.meegid.2020.104473 This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2020 Published by Elsevier.