Research Article Component Composition and Antimicrobial Activity of CO 2 Extract of Portulaca oleracea, Growing in the Territory of Kazakhstan Meruyert I. Tleubayeva , 1 Ubaidilla M. Datkhayev, 1 Mereke Alimzhanova, 2 Margarita Yu Ishmuratova , 3 Nadezhda V. Korotetskaya, 4 Raisa M. Abdullabekova, 5 Elena V. Flisyuk, 6 and Nadezhda G. Gemejiyeva 7 1 Department of Organization, Management and Economics of Pharmacy and Clinical Pharmacy, School of Pharmacy, Asfendiyarov Kazakh National Medical University, Almaty, St.Tole Bi, 88,050000, Kazakhstan 2 Department of Analytical, Colloidal Chemistry and Technology of Rare Elements, Al-Farabi Kazakh National University, Almaty, Al-Farabi Ave, 71, 050040, Kazakhstan 3 Department of Botany, E. Buketov Karaganda University, Karaganda, St. University, 28, 100028, Kazakhstan 4 Test Facility Management, JSC Scientific Center for Antiinfectious Drags, Almaty, Al-Farabi Ave, 75 A, 050060, Kazakhstan 5 Department of Pharmaceutical Disciplines and Chemistry, Medical University of Karaganda, Karaganda, St. Gogol, 40, 100008, Kazakhstan 6 Department Technology of Drags Forms, Saint-Petersburg State University of Chemical and Pharmaceuticals, St. Petersburg, St. Popova, 14, 197376, Russia 7 Laboratory of Plant Resources, Institute of Botany and Phyto-Introductions, Almaty, St. Timiryazev, 36 D, 050040, Kazakhstan Correspondence should be addressed to Meruyert I. Tleubayeva; meruert_iliasovna@mail.ru Received 11 July 2020; Revised 2 January 2021; Accepted 9 January 2021; Published 22 January 2021 Academic Editor: Jacek Karwowski Copyright©2021MeruyertI.Tleubayevaetal.isisanopenaccessarticledistributedundertheCreativeCommonsAttribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Inthemedicineofmanycountries,theuseofherbalhealingagentsincludedasignificantcontributiontoimprovinghumanhealth and well-being. Many antibiotics have been widely used to treat infectious diseases caused by various pathogenic bacteria. However,increasedmultidrugresistancehasledtoincreasedseverityofdiseasescausedbybacterialpathogens.Bacteriaremain themaincausativeagentsofdiseasesthatcausehumandeath,eveninthepresentday.iscausepromptedscientiststoinvestigate alternative new molecules against bacterial strains. e significant interest for the study is Portulaca oleracea L. (family Por- tulacaceae),awidespreadannualplantusedinfolkmedicine.us,theproductionandstudyofCO 2 extractof Portulaca oleracea isanactualproblem. Methods.RawmaterialswerecollectedfromAlmatyandZhambylregions(SoutheastandSouthKazakhstan) in phase flowering. Portulaca oleracea herb’s CO 2 extract was obtained by subcritical carbon dioxide extraction (installation of carbon dioxide flow-through extraction- 5L). e Wiley 7 th edition and NIST’02 library were used to identify the mass spectra obtained. e antimicrobial activity study was conducted by the micromethod of serial dilution and disco-diffuse method. Standard test strains of microorganisms were used: Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538-P, Candida albicans ATCC 10231, and Escherichia coli ATCC 8739. Results. e use of carbon dioxide extraction (further CO 2 extract) is a promising direction of obtaining total medicinal substances containing biologically active substances, from fractions of volatile estersofvariouscompositionandfunctionalpurposeuntilafractionoffattyacidsandfat-solublevitamins.Inthecurrentstudy, weobtainedCO 2 extractatsubcriticalconditionsfromabovegroundorgansof Portulaca oleracea andinvestigatedthecomponent compositionforthefirsttime.From41to66componentswereidentifiedinthecompositionof Portulaca oleracea‘sCO 2 extract. Studies of antimicrobial activity showed that CO 2 extract of Portulaca oleracea had the expressed effect against clinically sig- nificantmicroorganismssuchas Escherichia coli, Staphylococcus aureus, Bacillus subtilis,and Candida albicans. Conclusions.is studyshowedthatCO 2 extractof Portulaca oleracea’srawmaterialcontainedbiologicalactivecompoundsexhibitingasignificant antimicrobial effect. Hindawi e Scientific World Journal Volume 2021, Article ID 5434525, 10 pages https://doi.org/10.1155/2021/5434525