ANTIBACTERIAL ACTIVITY OF PLANT BIOSURFACTANT EXTRACT FROM SAPINDUS MUKOROSSI AND IN SILICO EVALUATION OF ITS BIOACTIVITY Short Communication INDIRA P. SARETHY 1 *, NIYANTA BHATIA 1 , NIDHI MAHESHWARI 2 1* Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, NOIDA-201307, India, 2 Received: 06 Jun 2015 Revised and Accepted: 20 Aug 2015 Ishan Herbotech International, D-10, Export Promotion Industrial Park, Site-V, Kasna, Greater Noida 201310 Email: indirap.sarethy@jiit.ac.in ABSTRACT Objective: Natural biosurfactants can replace synthetic surfactants and find applications in cosmetic products. Saponin extracts from Sapindus mukorossi fruits have emulsifying properties and have traditionally been used in hair care treatments. Their utility can be enhanced by studying their antimicrobial activity against common skin and other microorganisms. Methods: Aqueous biosurfactant extracts were prepared from fruits of S. mukorossi. Concentrated and diluted extracts were tested for antimicrobial activity against Micrococcus luteus, Brevibacterium linens, Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli and Pseudomonas fluorescens by the well diffusion method and measuring the zone of inhibition. In silico biological activity of different saponins present in S. mukorossi was studied using the software Prediction of Activity Spectra for Substances (PASS). Results: Concentrated extracts were most active against all the target microorganisms. Gram positive organisms were inhibited more than Gram negative ones. Diluted extracts produced comparable inhibition zones, suggesting that the extent of dilution does not affect the antimicrobial activity further. In silico evaluation showed that major saponin types (Sapindoside B, Sapinmusaponin A, Sapinmusaponin F and Sapinmusaponin N) had antibacterial activity with probable activity to probable inactivity (Pa>Pi) values less than the threshold level of 0.7. Conclusion: Biosurfactant (saponin) extracts from S. mukorossi, can be included in herbal care products not only for their emulsifying properties, but also for their antimicrobial effect. While in silico study showed less than threshold level of antibacterial activity, the combination of all these saponin types together probably contributed to the synergistic antibacterial activity. Keywords: Sapindus mukorossi, Biosurfactant, Saponin, Antimicrobial. Saponins are glycosides and have predominantly been studied from plant sources with some from animal sources [1]. While structurally diverse, they share the properties of hemolysis and foaming [2]. Surface activity, resulting in foaming, is due to the saponins. They show amphiphilic characteristics with sugar moiety and hydrophobic sapogenin. Their surfactant properties make them interesting for applications wherein they can potentially replace synthetic surfactants. Saponins find applications in pharmaceutical, food and cosmetic industries for their emulsifying properties [3]. Due to increased awareness of environmental safety, there has been growing interest in such natural surfactants from microbial and plant sources in recent times [4, 5]. Saponins have been traditionally used from Sapindus mukorossi, Quillaja bark, Balanites aegyptica and Fagonia indica [6-8]. The soap nut tree or Sapindus commonly grows in tropical and sub- tropical regions. The extract from the fruits (ritha) has been traditionally used in India for washing and hair care products. The pericarp of the fruits of Sapindus contains 6-10% saponins [9]. While many studies have reported the structural and functional properties of purified saponin, Ghagi et al., [10] have shown that emulsification properties of the crude saponin extract is similar to that of purified extracts, thereby potentially bringing down production costs. The biosurfactant extracts from Sapindus can be used in place of synthetic surfactants in products such as shampoos, soaps and other cosmetics. Being natural, apart from eco-friendly aspects, it is important to also understand their antimicrobial activities. Such characteristics would enhance their utility. Hence, the main objective of the present study was to evaluate the antimicrobial properties of plant biosurfactant extracts prepared from fruits of S. mukorossi. To our knowledge, comprehensive studies on the effect of biosurfactant from S. mukorossi for activity against common skin microorganisms have not been carried out. Our report presents the results of a study on its antibacterial activity against such skin- inhabiting and other bacteria. Further, in silico studies were performed on saponins from this plant to evaluate their antibacterial and other bioactivity potential. The fruits of S. mukorossi were soaked in water overnight (a ratio of 400g fruits to 1L water) and then boiled till it became soft. Aqueous extract was prepared from this softened fruit using a hydrostatic pressure machine. Concentrated form of the extract as well as 1:1 and 1:2 dilutions were prepared with water as diluent and used for studying antimicrobial activity. Agar-well diffusion method was used for this purpose. Mueller- Hinton agar was prepared, sterilized and poured into petri dishes. Six target organisms (obtained from Microbial Type Culture Collection-MTCC, Chandigarh) were used-Micrococcus luteus (MTCC- 106), Brevibacterium linens (MTCC-268), Bacillus subtilis (MTCC- 121), Staphylococcus epidermidis (MTCC-435), Escherichia coli (MTCC-1679) and Pseudomonas fluorescens (MTCC-2421). The target organisms (100 µl) were spread plated and 40 µl of each sample was loaded in triplicates in wells. Sodium Dodecyl Sulfate (SDS), as synthetic surfactant, at a concentration of 0.1% was used as positive control. The plates were incubated at 37 o To predict the biological activity profile of different saponins present in S. mukorossi, Prediction of Activity Spectra for Substances (PASS) software was used [11]. S. mukurossi contains a mixture of different saponins with Sapindoside B (oleanane type) as one of the major constituents [12]. The structures of Sapindoside B and other saponins such as Sapinmusaponin A (dammarane type), F (tricullane type) and N (oleanane type), obtained from PubChem or other literature, were used as input for PASS as per instructions and their biological activity predicted. C overnight and observed for zones of inhibition. The diameters of the clear zones were measured. All experiments were performed in triplicates and experiments repeated thrice. The data were expressed as mean±standard deviation. Data was subjected to one way analysis of variance International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 7, Issue 10, 2015 Innovare Academic Sciences