Open Journal of Veterinary Medicine, 2017, 7, 111-119 http://www.scirp.org/journal/ojvm ISSN Online: 2165-3364 ISSN Print: 2165-3356 DOI: 10.4236/ojvm.2017.79011 Sep. 30, 2017 111 Open Journal of Veterinary Medicine Detection of Antibody in Dogs with Blastomycosis Using Blastomyces dermatitidis Yeast Phase Lysate Antigens Bryn C. Kennell 1 , Gene M. Scalarone 1 , Lilliana Hoyos-Carvajal 2 , Moytri RoyChowdhury 1* 1 Department of Biological Sciences, Idaho State University, 650 Memorial Dr., Gale Life Sciences Building, Pocatello, ID, USA 2 Universidad Nacional de Colombia, Sede Medellín, Colombia Abstract The objective of our study was to compare two B. dermatitidis yeast phase ly- sate antigens [ERC-2, dog Wisconsin; 85, soil Georgia, ATCC 56,920] for de- tecting antibody in 38 serum specimens [pre-treatment, 30-day, and 60-day post treatment] from dogs with diagnosed blastomycosis. The mean absor- bance values obtained with the two antigens (N = 38) were ERC-2 = 2.359 and 85 = 2.189. The mean absorbance values when the sera were divided into the three treatment groups were as follows pre-treatment: Isolate ERC-2 had an absorbance value of 2.418; Isolate 85 had an absorbance value of 2.688, 30-day post treatment: ERC-2 had an absorbance value of 2.452; 85 had an absor- bance value of 2.303 and 60-day post treatment: ERC-2 had an absorbance value of 2.150; 85 had an absorbance value of 2.073 with the mean absorbance values of all treatment groups were ERC-2: 2.229 and 85: 2.141. This study in- dicates the potential for further evaluations of the two lysate antigens with re- gard to antibody detection in dog sera with the ERC-2 reagent slightly more reactive than the 85 lysate antigen. Keywords Blastomyces Dermatitidis, Yeast Phase Lysate Antigen, Blastomycosis 1. Introduction Blastomycosis is a systemic mycosis that can prove fatal, particularly among the immunocompromised. Blastomycosis is caused by the inhalation of the thermally dimorphic fungal agents Blastomyces dermatitidis. A common re- servoir of B. dermatitidis is moist soil and decaying matter, especially in en- How to cite this paper: Kennell, B.C., Scalarone, G.M., Hoyos-Carvajal, L. and RoyChowdhury, M. (2017) Detection of Antibody in Dogs with Blastomycosis Us- ing Blastomyces dermatitidis Yeast Phase Lysate Antigens. Open Journal of Veteri- nary Medicine, 7, 111-119. https://doi.org/10.4236/ojvm.2017.79011 Received: July 10, 2017 Accepted: September 27, 2017 Published: September 30, 2017 Copyright © 2017 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access