JOURNAL OF MEDIOCAL CORNVUS 2(7): 18-29 ISSN:____-____ www.gulfpublishers.com 18 Immunoinformatics Approach for Multi Components Vaccine Against Human Immunodeficiency Virus *Adeoti O.M. 1, 2, 3 , Jimoh A.O. 1 , Adeoye K.A 1, 3 , Olaoye V.A. 1 1. The Oke- Ogun Polytechnic, Saki Oyo State Nigeria, Department of Science Laboratory Technology, Microbiology option 2. Ladoke Akintola University of Technology, Department of Pure & Applied Biology, Ogbomoso 3. Department of Zoology, Parasitology Unit, University of Ibadan, Nigeria. 4.Cellular Parasitology Unit, Department of Zoology, University of Ibadan, Nigeria Abstract: The human immunodeficiency virus (HIV) is a single-stranded RNA virus which is responsible for the cause of Acquired Immune Deficiency Syndrome (AIDS). An urgent need arises to establish antigen-based immunodiagnostic for earlier monitoring of HIV and the development of vaccines. Therefore an efficient vaccine design against HIV is necessary which is achievable with the use of the most current bioinformatics tools. In this study, immune-informatics approach was used to design a multi-component vaccine against HIV. The present study aims to choose and analyze different epitopes of Gag_Hv1h2, Rev_Hv1h3, Pol_Hv1b1, Pol_Hv1n5, Pol_Hv2ro, Pol_Hv1h2, Nef_Hv1br, Env_Hv1h2, Env_Hv1br, Vif_Hv1n5, Vif_Hv1h2, Vpr_Hv1h2, Vpu_Hv1br, Vpu_Hv1h2, Vpr_Hv1br, Tat_Hv1h2, Tat_Hv1br, Zep2_Mouse, Zep2_Human, Zep2_Rat using bioinformatics analysis and software. B-cell epitopes were predicted using IEDB and NetMHC webserver was used to the prediction of binding of peptides to MHC Class-I and Class II. Physicochemical analysis of vaccine showed that the molecular weight of candidate vaccine is <50000kDa with the half-life of 30 hours in mammalian reticulocytes, greater than 20 hours for yeast, and greater than 10 hours for E.coli. The chemicals formula of the vaccine was C1933H3139N653O615S9, and the theoretical pl of the candidate vaccine was 10.36. There are 400 amino acids in the protein structure. The aliphatic index is 58.00, the stability index is computed to be 41.72, and then it is classified as unstable proteins. The vaccine has two transmembrane helix, therefore no expression difficulties are predicted in the production of the vaccine. Ramachandran plot analysis by pro check showed that 92.5% of residues are in most favored regions 7.5% of residues are additional allowed regions and 0.0% of residues are in disallowed regions. The vaccine may activate humoral and cellular immune responses against of Human Immunodeficiency Virus. However, the vaccine was evaluated as non-allergenic, antigenic, soluble, and with an appropriate molecular weight and isoelectric point. This novel oral vaccine design could be a good vaccine candidate against HIV. Keywords: Immunoinformatics, Ramachandra Plot, Z-score, Physicochemical, Multi-epitopes, Vaccine. 1. INTRODUCTION The human immunodeficiency virus (HIV) may be a single- stranded RNA virus that is responsible to the explanation for acquired immune deficiency syndrome (AIDS). AIDS is defined either in terms of when CD4+ T cell counts below 200 cells per microliter or the occurrence of specific diseases in association with an HIV infection and the immunity of the physical body is weak. [1]. during a recent report suggested by the Centers for Disease Control and Prevention (CDC) show that 1,148,200 Americans aged 13 and older were living with HIV [2]. However, within the 2008-09 National AIDS Control Organization has reported that 2.39 million people accept HIV/AIDS in India [3]. While a newer investigation by the Million Death Study Collaborators within the British Medical Journal estimated that 1.4-1.6 million people suffer with AIDS [4]. There's an urgent need for controlling of AIDS by developing or producing rapid, eco-friendly, and price effective immunodiagnostic capability. Therefore, two sorts of HIV are characterized including HIV-1 and HIV-2. HIV-1 is that the virus that was initially discovered and thought of to be more virulent, which caused the bulk of HIV infections worldwide [5]. The lower infectivity of HIV-2 compared to HIV-1 implies that fewer of these exposed to HIV-2 are going to be infected per exposure, due to its relatively poor capacity for transmission; HIV-2 is essentially confined to West Africa [6]. The culture of HIV is in laboratory is difficult and time consuming process; thus, variety of immune-informatics tool are developed for prediction of T and B cells epitopes. Vaccine design within the pre-omics era depended heavily on biochemical and immunological techniques like phage display library, overlapping peptides, ELISA, immunohistochemistry, immunofluorescence, radioimmunoassay, Western blotting, NMR spectroscopy, X-ray crystallography of antibody-antigen structure, and attenuation of untamed type pathogens by random mutation and serial passage, a process that's very REVIEW ARTICLE Received: 16-07-2021 Accepted: 25-07-2021 Published: 26-07-2021 * Corresponding author:( Adeoti O.M.) Published online at http://gulfpublishers.com/journal/1 Copyright © 2020 The Author(s). Published by Gulf Publishers This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/