October 2019 · Volume 8 · Issue 10 Page 4034 International Journal of Reproduction, Contraception, Obstetrics and Gynecology Wulandari D et al. Int J Reprod Contracept Obstet Gynecol. 2019 Oct;8(10):4034-4039 www.ijrcog.org pISSN 2320-1770 | eISSN 2320-1789 Original Research Article Levels of molecular angiogenic and antiangiogenic in pregnant women with risk of preeclampsia Diah Wulandari 1 *, Mohamad Sulchan 2 , Syarief Thaufik Hidayat 3 INTRODUCTION Preeclampsia is a specific condition in pregnancy which is characterized by the presence of placental dysfunction and maternal response due to systemic inflammation along with endothelial activation and coagulation. 1 The reported incidence of preeclampsia in 2013 in Indonesia was 128,273/year or estimated around 5.3%. Maternal mortality caused by preeclampsia was reported to be 29,000 and as many as 25.8% of the Indonesian population aged more than 18 years old were suffered from hypertension, whereas 6-10% of them were happened during pregnancy. 2 Preeclampsia is indeed called as a "disease of theories," because the pathogenesis of preeclampsia has not been well understood. Some of these theories are placental ischemia, oxidative stress and free radicals, and the ABSTRACT Background: Angiogenic and antiangiogenic imbalances play a major role in the pathogenesis of preeclampsia. Increased production of sFlt-1 by the placenta causes free circulating PIGF and VEGF concentrations to lower because it is bound by sFlt-1. Measuring levels of angiogenic and antiangiogenic proteins as biomarkers indicates placental dysfunction and distinguishes preeclampsia from other disorders. This study aims to analyze the levels of angiogenic and antiangiogenic molecules in pregnant women at risk for preeclampsia. Methods: The study with a cross-sectional design was carried out in 11-15 weeks gestational age whom had a risk of preeclampsia with 30 samples in primary health care starting April-August 2018. Blood serum was measured by molecular levels of VEGF, PlGF, sFlt-1, and sFlt-1/PlGF ratio using the ELISA method. Data analysis used Pearson product moment test. Results: The mean of VEGF levels are 15.5±21.6, PlGF 89.7±55.5, sFlt-1 11519.4±5126.0 and the ratio sFlt-1/PlGF 166.7±102.1. Correlation value of risk factors for preeclampsia with molecular levels of VEGF r= -0.05; p = 0.76, PlGF r= -0.21; p = 0.26, sFlt r= 0.01; p =0.99 and ratio sFlt-1/PlGF r = 0.10; p = 0.58. Conclusions: The higher the total score of preeclampsia risk factor, the lower the molecular level of VEGF and PlGF is. Moreover, the higher the total score of preeclampsia risk factor, the higher the molecular level sFlt-1 and the sFlt- 1/PlGF ratio is. There are no significant correlation between total score of preeclampsia risk factor and levels of molecule VEGF, PlGF, sFlt-1 and sFlt-1/PlGF ratio. Keywords: Preeclampsia, PlGF, Risk factors, sFlt-1, VEGF 1 Department of Midwifery, Universitas Gadjah Mada, Yogyakarta, Indonesia 2 Departement of Nutrition Science, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia 3 Departement of Obstetrics and Gynecology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia Received: 02 August 2019 Revised: 03 September 2019 Accepted: 11 September 2019 *Correspondence: Dr. Diah Wulandari, E-mail: diah_wulandari@ugm.ac.id Copyright: © the author(s), publisher and licensee Medip Academy. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. DOI: http://dx.doi.org/10.18203/2320-1770.ijrcog20194376