Meera Chandradatt Singh & K.N.Gujar. Int. Res. J. Pharm. 2018, 9 (11) 164 INTERNATIONAL RESEARCH JOURNAL OF PHARMACY www.irjponline.com ISSN 2230 – 8407 Research Article PREPARATION AND EVALUATION OF NUTRACEUTICAL FORMULATIONS OF LEAVES OF TRIGONELLA FOENUM GRAECUM, CORIANDRUM SATIVUM, RAPHANUS RAPHANISTRUM AND ANETHUM GRAVEOLENS Meera Chandradatt Singh * 1 , K.N.Gujar 2 1 PhD Scholar, PAHER’s Pacific University , Debari, Udaipur Rajasthan, Assistant Professor at S.T.E.S’s S.K.N. College of Pharmacy, Kondhwa , Pune 48 , Maharashtra, India 2 Principal and Professor, S.T.E.S’s Sinhgad College of Pharmacy, Vadgaon , Pune, Maharashtra, India *Corresponding Author Email: meerasingh2109@gmail.com Article Received on: 10/10/18 Approved for publication: 19/11/18 DOI: 10.7897/2230-8407.0911277 ABSTRACT Introduction - In recent years there is a growing market for nutraceuticals which are proven to have health benefits and preventive in certain diseases. Nutraceuticals are developed and are being developed to target specific disease. Scope-The nutraceutical market escalated and has reached to its maturity in USA, Europe and in Japan. In India it’s in infancy, though the scope is huge, especially owing to the vast variety of vegetables, fruits and grains which are grown in India Objective-This research has made an attempt to evaluate four rich green leafy vegetables namely Trigonella foenum graecum, Coriandrum sativum, Raphanus raphanistrum and Anethum graveolens for nutraceutical, prebiotic and antioxidant potential individually and in combination. The results were encouraging which prompted preparation of two formulations of the four leafy vegetable mixture. Methodology- The whole powder mixture was analysed for nutrient content, prebiotic and antioxidant potential. The mixture was designed into dry powder health drink formula to be mixed with warm water. The ethanol extract of four leaves mixture was obtained to get more concentrated nutrients and was developed into capsule dosage form. Both formulations complied with the required in-vitro pharmaceutical parameters of evaluation. Conclusion-Nutraceuticals developed under this research are rich in antioxidants, essential nutrients, proteins, soluble fibres and thus can have applications in treatment and prevention of many illnesses that can be helped with prebiotic and antioxidant content. Keywords: Nutraceutical, Trigonella foenum graecum, Coriandrum sativum, Raphanus raphanistrum , Anethum graveolens, prebiotic, antioxidant. INTRODUCTION Today foods are not intended to only satisfy hunger and to provide necessary nutrients for humans, but also to prevent nutrition- related diseases and improve physical and mental well-being of the consumers. In this regard, nutraceuticals play an outstanding role and the market for them is increasing. 1, 2 Nutraceuticals are a broad umbrella term used to describe any product derived from food sources that provides extra health benefits in addition to the basic nutritional value found in foods. The term Nutraceutical is a hybrid of nutrition and pharmaceutical technology, coined in 1989 by Stephen Defelice. 3 Nutraceutical-rich vegetables and fruits are an important component of a healthy diet. There has been an explosion of consumer interest in the health enhancing role of physiologically- active specific nutraceuticals. 4 Individually Trigonella foenum graecum (Fenugreek leaves) Coriandrum sativum ( coriander leaves) Raphanus raphanistrum ( Radish leaves) and Anethum graveolens ( Dill leaves) have been reported to contain rich macro and micro nutrients. Under this research these four leaves in dried powder form are used in equal proportion as the basic nutraceutical mixture. The combination increases nutraceutical contents, prebiotic potential and antioxidant properties which are marker qualities of any nutraceutical. Two teaspoonful of dry powder of leafy vegetable is actually equal to one vegetable serving. When cooked all vegetable loose some nutrition. In the nutraceutical formulations the chances of nutrition preservation are more, which serves the larger purpose of health improvement and prevention and cure of diseases. 5 Trigonella foenum graecum -Fenugreek leaves reported to contain - 4.4% protein, 0.9% fat, 1.5% minerals, 1.1% fiber, and 6% carbohydrates. The minerals and vitamins include calcium, zinc, iron, phosphorous, riboflavin, carotene, thiamine, niacin, vitamin C and β-carotene about 19 mg/100 g. The reported medical benefits are that it improves heart function as prevents atherosclerosis, lowers cholesterol level, helpful in anaemia as rich in iron along with prebiotic potential 6,7,8,9 Coriandrum sativum,-Coriander leaves reported to contain - Crude protein 10.61 %, Crude fiber 5.97 %, Minerals- Calcium 2.11%, Potassium 4.29% Iron 0.091% The reported medical benefits are that it lowers cholesterol, stimulates the insulin secretion, antiseptic, anti-inflammatory, along with prebiotic potential 10,11,12 Raphanus raphanistrum -Radish leaves. The green parts of a radish contain more nutrients than the entire radish itself and are eaten as vegetable in India. It contains minerals like iron, calcium, folic acid, vitamin C and phosphorous. It’s proximate analysis – shows to contain Crude Fat 4.03 %, Protein 3-3 % , Crude fibre 13.5%, Ash 20.63%, Carbohydrate 12.16%, and antioxidants 326.80± mg/g. Medical benefits include –It treats constipation, increases immunity and reduces fatigue, It is diuretic and anti- scorbutic, it treats piles, helps to prevent diabetes:, and it is detoxifying agent: 13,14