International Journal of Innovative Research in Medical Science (IJIRMS) Volume 03 Issue 04 April 2018, ISSN No. - 2455-8737 Available online at - www.ijirms.in 1976 Indexcopernicus value - 64.48 © 2018 Published by IJIRMS Publication Bacteriological Profile of PUS/ WOUND SWAB and Antimicrobial Susceptibility of Staphylococcus Aureus Isolated From PUS & WOUND SWAB of Indoor Patients of Tertiary Care Hospital, Durg, Chhattisgarh India Nandkishor Bankar 1 , Archana Wankhade 2 , R B Brahmane 3 , Riddhi Hathiwala 4 , Dhruba Hari Chandi 5 1,4,5 Assistant Professor, 2 Associate Professor, 3 Professor and HOD Department of Microbiology CCM Medical College Durg (CG) Address for Correspondence Nandkishor Bankar Assistant Professor Dept. of Microbiology, CCM Medical College, Kachandur, Durg (CG) Email: drbankarnj28@gmail.com Abstract Wound infection can be caused by variety of organisms like bacteria, virus, fungi and protozoa and may co-exist as poly microbial communities. Even though the bacterial profile of pus samples in many studies remain the same, the antibiotic resistance pattern of these isolates has shown a lot of variations. This study was conducted in the department of microbiology of a private tertiary care hospital CCM Medical College Durg, Chhattisgarh over a period of one year from January 2017 to December 2017. Antibiotic sensitivity testing of Staphylococcal isolates was performed by modified Kirby Bauer’s disc diffusion method. Tota l of 387 samples were processed for aerobic culture and sensitivity, of which in 304 (78.55%) samples single growth was observed. Of the total 304 isolates, 274 isolates were from surgical units and 30 isolates from medicine units. Isolation of Gram positive and Gram negative organisms was 144 (47.36%) and 158 (51.97%) respectively and 2 (0.65%) species were Candida. Antibiotic susceptibility testing was done on 104 Staphylococcus aureus isolates for MRSA detection and antibiotic resistance pattern. 93.3% isolates were resistant to penicillin followed by cotrimoxazole (67.3%) and erythromycin (50%). Resistance to linezolid was observed in 2.9% cases while vancomycin and teicoplanin showed 1.9 % and 6.7% resistance. 26.9 % isolates were MRSA. This study gives an outline of antibiotic susceptibility of Staphylococcal isolates to formulate the local antibiotic policy to start the appropriate empirical antibiotic treatment before the culture reports are available. Introduction Pyogenic infections are characterized by local and systemic inflammation, formation of pus, generally caused by one of the pyogenic bacteria, which result in the aggregation of dead leukocytes as well as pyogenic bacteria commonly known as pus, “a white to yellow fluid”. [1,2] Wounds are result of loss of intact skin due to injury caused by external forces such as surgical wounds, burns, bites, abrasions, minor cuts and more severe traumatic wounds such as lacerations and crush or gunshot injuries such discontinuity in skin is good environment for microbial colonization as there is presence of moisture, warmth and nutrition for their growth. Colonization with proliferation of bacterial flora may lead to wound infection which may be serious even sometimes lead to death. These may be endogenous or exogenous. A break in the skin can provide entry to the surface bacteria which thereby start multiplying locally. The body’s defense mechanism includes bringing immune cells into the area to fight against bacteria. Eventually, accumulation of these cells produces pus which is a thick whitish liquid. [3] In most of the studies it is seen that majority of the isolates are aerobes which includes Gram positive cocci like Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Enterococci and Gram negative bacilli like Escherichia coli, Klebsiella pneumoniae, Proteus and Pseudomonas. [4] Wound infection can be caused by variety of organisms like bacteria, virus, fungi and protozoa and may co-exist as poly microbial communities especially in wound margins and in chronic wounds [5] and in many Open Access Journal Research Article DOI: 10.23958/ijirms/vol03-i04/12