S1 SUPPLEMENTARY INFORMATION Development of an Automated Kinetic Profiling System with Online HPLC for Reaction Optimization Melodie Christensen,* a,c Folarin Adedeji, a Shane Grosser, a Kerstin Zawatzky, b Yining Ji, a Jinchu Liu, b Jon A. Jurica, a John R. Naber, a and Jason E. Hein* c *Email for M.C.: melodie.christensen@merck.com, Email for J.E.H.: jhein@chem.ubc.ca Contents 1. General Remarks ................................................................................................................................... 2 1.1. Chemical Suppliers ........................................................................................................................ 2 1.2. Equipment ..................................................................................................................................... 2 1.3. Analytical Methods ....................................................................................................................... 3 2. Experimental Procedures ...................................................................................................................... 4 2.1. Procedures for Automated Kinetic Profiling ................................................................................. 4 2.2. Procedures for Preparation of Reaction Components.................................................................. 6 2.3. Sample Chemspeed Autosuite Protocol for Automated Kinetic Profiling .................................... 9 2.4. Picture of Chemspeed Robot Deck ............................................................................................. 10 3. Characterization of New Compounds ................................................................................................. 11 4. Calibration Curves ............................................................................................................................... 15 a. Process Research and Development, Merck & Co., Inc., Rahway, NJ, USA b. Analytical Research and Development, Merck & Co., Inc., Rahway, NJ, USA c. Department of Chemistry, The University of British Columbia, Vancouver, BC, CA Electronic Supplementary Material (ESI) for Reaction Chemistry & Engineering. This journal is © The Royal Society of Chemistry 2019