Journal of Molecular Biology Research; Vol. 9, No. 1; 2019 ISSN 1925-430X E-ISSN 1925-4318 Published by Canadian Center of Science and Education 49 The Effect of Mixed Organic Solvents Extract of Trachyspermum ammi with Different Concentrations on the Body Weight of Mice Ali Akbar Moghadamnia 1 , Maria Hashemi 2 , Parvin Sajadi Kaboudi 3 , & Zahra Geraili 4 1 Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran 2 Department of Pharmacology, School of Medicine, Babol University of Medical Sciences, Babol, I.R Iran 3 Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran 4 Department of Statistic and Epidmiology, School of Medicine, Babol University of Medical Sciences, Babol, I.R Iran Correspondence: Parvin Sajadi Kaboudi, Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran. Tel: 98-911-114-8704, Email: psajadi@yahoo.com. Received: April 17, 2019 Accepted: May 10, 2019 Online Published: May 29, 2019 doi:10.5539/jmbr.v9n1p49 URL: https://doi.org/10.5539/jmbr.v9n1p49 Abstract Background and Aim. In traditional medicine, the use of Trachyspermum ammi, commonly known as 'Ajwain', is recommended to improve digestive function of the stomach and has effects on body weight. The present study aims to determine the effect different concentrations of mixed organic solvents extract (MOSE) of Trachyspermum ammi on mice compared with control group. Materials and Methods. This experimental-interventional study was performed on mice weighing an average of 21 gr, selected through random allocation method. The mice were divided into four groups of 24 (one control group and three case groups), receiving 0.5, 1 and 2% concentrations or density of MOSE of Trachyspermum ammi (0.001 mg/kg). First, the dried fruit of Trachyspermum ammi was powdered and then, it was extracted using various organic solvents including petroleum ether, chloroform, ethyl acetate and ethyl alcohol. The extract was fed to the mice for 14 days and their weight was controlled every day. Results. The hypothesis of diversity in the weight gain of mice among the four groups after 15 days was significant; weight gain in control group was significantly higher than other groups (p<0.05). In addition, comparing the weight gain of the mice that received different concentrations of MOSE of Trachyspermum ammi (0.5, 1 and 2%) did not show a significant difference (p<0.05). Conclusion. The use of Trachyspermum ammi plant can not be effect on body weight compared to the control group. This could not be a suitable way to weight change through diet. Keywords: Mixed organic solvents extract of Trachyspermum ammi, Mice, Weight gain 1. Introduction Trachyspermum ammi is a medicinal herb which is used to cure central nervous system (CNS) involvement, gastrointestinal disorders such as nausea, vomiting, abdominal cramps and loss of appetite, reduces the pain (Markesbery, 1997; Mohanty, 2017; Sivropoulou et al., 1996). This plant is broadly distributed and cultivated in many areas of Mediterranean countries and Iran, Afghanistan, Pakistan, Egypt, and India as well as European region(Bairwa, Sodha, & Rajawat, 2012). An annual herbaceous plant belonging to the highly valued medicinally serious family, Apiaceae. The past of this plant is not completely clear, but we know that in the traditional medicine it was prescribed to improve the digestive process or digestive diseases like as flatulence(Vasudevan, Vembar, Veeraraghavan, & Haranath, 2000). The most important materials obtained from Trachyspermum ammi are 35-50 percent thymol, 15-20 percent cymene and 30-40 percent α-pinene(Ishikawa, Sega, & Kitajima, 2001). Trachyspermum ammi (Apiaceae) against Setaria digitata worms has been assessed(Chahal, Dhaiwal, Kumar, Kataria, & Singla, 2017;