Panacea Journal of Medical Sciences 2023;13(2):299–304 Content available at: https://www.ipinnovative.com/open-access-journals Panacea Journal of Medical Sciences Journal homepage: http://www.pjms.in/ Original Research Article Surgical site infection: Clinico- bacteriological Profile and antibiogram in a tertiary care hospital in Kolkata Kabita Choudhury 1 , Swati Basu 1 , Swagata Ganguly Bhattacharjee 1 , Subhendu Sikdar 2, *, Sonia Deb 3 , Nourine Tabassum 1 1 Dept. of Microbiology, NRS Medical College & Hospital, Kolkata, West Bengal, India 2 Dept. of Microbiology, R. G. Kar Medical College & Hospital, Kolkata, West Bengal, India 3 Dept. of Microbiology, School of Tropical Medicine, Kolkata, West Bengal, India ARTICLE INFO Article history: Received 16-12-2021 Accepted 14-02-2023 Available online 31-07-2023 Keywords: Emergency surgery Serum albumin levels Wound site infection ABSTRACT Introduction: Surgical site infections (SSIs) are a major health care associated infections and possess a great challenge to clinicians. It increases duration of hospital stay, associated morbidity and mortality and treatment cost. For prompt and appropriate management, it is necessary to understand the appropriate microbial etiology and its antimicrobial susceptibility pattern. Aim: To determine the prevalence of SSI along with its clinico-bacteriological profile and antibiogram and to correlate with the risk factors. Materials and Methods: A cross-sectional study was conducted in the Department of Microbiology of Nil Ratan Sircar Medical College & Hospital, Kolkata for a period of one year (2020-2021). Post-operative surgical site infections within 30 days after surgery were included in the study. All samples were collected from clinically suspected SSI cases and processed in the lab as per standard laboratory protocol. Vitek®2 compact system (BIOMERIEUX) was used for further identification and antimicrobial susceptibility testing. All Gram negative pathogens resistant to 3rd generation cephalosporins were screened for ESBL and AmpC. Carbapenem resistant isolates were subjected to Carba NP test for carbapenemase production. Results and Analysis: Out of 6582 surgeries, clinically suspected SSIs were detected in 220 cases. Among these 220 patients, 186 patients were confirmed as SSI in the laboratory, SSI rate being 2.83%. In 62.27% cases, growth was mono-microbial. Growth of gram negative organisms were noted in 58.72% cases and gram positive growth was 38.53%. Staphylococcus aureus (57.14%) was the predominant isolates followed by Escherichia coli (35.94%). Diabetes mellitus, obesity and using prophylactic antibiotic >2 hours prior to surgery were found to be significantly associated. For gram positive organism, Vancomycin and Linezolid were found to be most susceptible antimicrobials and for gram negative organisms, it was Polymyxin B and Meropenem. Most of the gram negative organisms were ESBL producer (67.97%). Conclusion: Rapidly emerging multi drug resistant organisms are complicating the management of surgical site infection day by day and possessing significant burden to health care system. For implementation of appropriate antimicrobial policy, it is important to identify bacteriological profile and antibiogram in every hospital. This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. For reprints contact: reprint@ipinnovative.com * Corresponding author. E-mail address: subhendu.kolkata26@gmail.com (S. Sikdar). 1. Introduction Surgical site infection, a major public health problem, is the second most frequently reported health care associated infections worldwide. 1 Center of Disease Control (CDC), https://doi.org/10.18231/j.pjms.2023.058 2249-8176/© 2023 Innovative Publication, All rights reserved. 299