© 2015. Karagyozov T. H., Mammadova M. H., Asadova S. Sh. & Azizov I. V. This is a research/review paper, distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License http://creativecommons.org/licenses/by- nc/3.0/), permitting all non commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Global Journal of Science Frontier Research: C Biological Science Volume 15 Issue 6 Version 1.0 Year 2015 Type : Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc. (USA) Online ISSN: 2249-4626 & Print ISSN: 0975-5896 A New Approach to Biotechnology of Saffron (Crocus Sativus L.) By Karagyozov T. H., Mammadova M. H., Asadova S. Sh. & Azizov I. V. Institute of Botany of Nat. Acad. Sci. of Azerbaijan, Azerbaijan Abstract- To develop effective approaches for biotechnological propagation of saffron(Crocus sativus L.)in vitro methods for the induction of morphogenesis and organogenesis were used. Factors influencing morphogenesis and organogenesis of saffron were considered. By using a temperature gradient under in vitro conditions was obtained de novo from 10 to 25 microcorms. Some issues related to biotechnology of saffron were elucidated. The effect of the temperature factor and gibberellin on the efficiency of callus formation and morphogenesis in vitrowerediscussed. The methodical approaches to increase the efficiency of morphogenesis and organogenesis of Crocus sativus L. in in vitro conditions were offered. Keywords: saffron, Crocus sativus L., in vitro, morphogenesis, temperature qradient. GJSFR-C Classification : FOR Code: 270499 ANewApproachtoBiotechnologyofSaffronCrocusSativusL Strictly as per the compliance and regulations of :