1 TXN, a Xanthohumol Derivative, Attenuates High-Fat Diet Induced Hepatic 1 Steatosis by Antagonizing PPARγ 2 Yang Zhang 1* , Gerd Bobe 2 , Cristobal L. Miranda 3 , Malcolm B. Lowry 4 , Victor L. Hsu 5 , 3 Christiane V. Löhr 6 , Carmen P. Wong 1 , Donald B. Jump 1 , Matthew M. Robinson 7 , Thomas J. 4 Sharpton 8 , Claudia S. Maier 9 , Jan F. Stevens 3 , Adrian F. Gombart 10* 5 1 School of Biological and Population Health Sciences, Nutrition Program, Linus Pauling 6 Institute, Oregon State University, Corvallis, OR 97331, USA 7 2 Department of Animal Sciences, Linus Pauling Institute, Oregon State University, Corvallis, 8 OR 97331, USA 9 3 Department of Pharmaceutical Sciences, Linus Pauling Institute, Oregon State University, 10 Corvallis, OR 97331, USA 11 4 Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA 12 5 Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331, 13 USA 14 6 Department of Biomedical Science, Carlson College of Veterinary Medicine, Corvallis, OR 15 97331, USA 16 7 School of Biological and Population Health Sciences, Kinesiology Program, Oregon State 17 University, Corvallis, OR 97331, USA 18 8 Department of Microbiology, Department of Statistics, Oregon State University, Corvallis, OR 19 97331, USA 20 9 Department of Chemistry, Linus Pauling Institute, Oregon State University, Corvallis, OR 21 97331, USA 22 10 Linus Pauling Institute, Department of Biochemistry and Biophysics, Oregon State University, 23 Corvallis, OR 97331, USA 24 * Authors to whom correspondence should be addressed. 25 (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint this version posted January 25, 2021. ; https://doi.org/10.1101/2021.01.11.426043 doi: bioRxiv preprint