_____________________________________________________________________________________________________ *Corresponding author: E-mail: ushachidu@yahoo.com; Journal of Pharmaceutical Research International 33(28B): 160-168, 2021; Article no.JPRI.68295 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Evaluation of Sensitivity, Specificity, and Cost- Effectiveness of Paper-Based Microfluidics for DNA Diagnostics of Malaria versus Nucleic Acid Test (NAT) Versus Rapid Diagnostic Tests (RDT) in Resource-Limited Settings: A Protocol Usha Adiga 1* and Tirthal Rai 1 1 Department of Biochemistry, Nitte (Deemed to be University), KS Hegde Medical Academy (KSHEMA), Mangalore, India. Authors’ contributions This work was carried out in collaboration between both authors. Author UA designed the study, wrote the protocol and wrote the first draft of the manuscript. Author TR managed the literature searches. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i28B31550 Editor(s): (1) Dr. Mohamed Salem Nasr Allah, Weill Cornell Medical College, Qatar. Reviewers: (1) Rini Hamsidi S. Farm, Universitas Airlangga, Indonesia. (2) Shravan Kumar Paswan, All India Institute of Medical Sciences, India. Complete Peer review History: http://www.sdiarticle4.com/review-history/68295 Received 01 March 2021 Accepted 08 May 2021 Published 13 May 2021 ABSTRACT Objective: The objective of the study is to compare three techniques, routinely used rapid diagnostic tests (lateral flow immune chromatography) versus nucleic acid amplification test (NAT) versus Paper-based microfluidics for DNA diagnostics of Malaria, in terms of their sensitivity and specificity as diagnostic tests in detecting malarial infection among febrile illnesses, suspected of malaria, as well as to compare their cost-effectiveness. Methodology: Three seventy febrile cases suspected of malaria with negative results with RDT will be screened by real-time PCR and DNA microfluidics techniques, sensitivity and specificity of these as screening tests will be compared. The number of extra positive cases detected by NAT gives us the yield. Cost-effectiveness analysis will be done by calculating the incremental cost-effectiveness ratio (ICER) and average cost-effectiveness ratio (ACER) for the tests. Statistical Analysis: Statistical analysis will be done using SPSS version 21. Sensitivity, specificity, Positive predictive values will be computed. Comparison of sensitivity and specificity of Review Article