Volume 58 Issue 2 (February 2024) 293 RESEARCH ARTICLE Indian Journal of Animal Research, Volume 58 Issue 2: 293-297 (February 2024) Effect of Copper and Zinc Supplementation on Antioxidants and Biochemical Status of Osmanabadi Goats S. Manimaran 1 , P.M. Kekan 1 , S.B. Daware 1 , A.K. Wankar 1 , V.K. Munde 2 , K.K. Khose 3 , P.M. Bhagade 1 10.18805/IJAR.B-4926 ABSTRACT Background: Copper and zinc in animal diets significantly impact livestock productivity. Zinc is not stored in the body and must be supplemented regularly to maintain optimal immuno-physiological function in animals. Many factors restrict the bioavailability of zinc and its absorption in the intestine. Cu deficiency is common in feed ingredients. Feeding copper levels in the diet is also essential for immune system optimization since copper inhibits the development of metabolic and oxidative stress in dairy cows. Methods: Experimental animals (n=18) were equally divided into three groups with an average body weight 29 kg. T0 was control group with basal diet, T1 group supplemented with copper sulphate (100 mg/goat/day+Basal diet) and T2 group supplemented with zinc oxide (40 mg/goat/day+Basal diet) for 90 days. Blood samples were collected at fortnight intervals and estimated using standard protocols. Result: Total antioxidant capacity (TAC) Superoxide dismutase (SOD) and Catalase were significantly higher in zinc supplemented group followed by copper supplemented group. Thiobarbituric acid reactive substances (TBARS) were significantly lower in the zinc and copper supplemented groups compared to control group. Glucose concentration showed no significant change. Total cholesterol was significantly lower in the copper supplemented group. Total proteins, albumin and triglycerides were significantly higher in the zinc supplemented group than in other groups. Key words: Biochemical, Catalase, Copper sulphate, Superoxide dismutase, Thiobarbituric acid reactive substance, Total antioxidants, Zinc oxide. I NTRODUCTION Like other nutrient requirements, mineral requirements for small ruminants are not stable. Mineral requirements for goats depend upon age, sex stage and level of production. Trace mineral deficiencies are many times difficult to detect because of their symptoms that are less evident (small reductions in their average daily gains and decreased production). Zinc and copper are the second and third most essential trace minerals next to iron. Copper (Cu) and zinc (Zn) in animal diets significantly impact livestock productivity (Arangasamy et al., 2018). Zinc is a nutritionally important trace element for goats since it is required for optimal feed intake and nutrient utilization, food metabolism and immunological competence (Neathery et al., 1973). Zinc is not stored in the body and must be supplemented regularly to maintain optimal immuno-physiological function in animals (Spears and Kegley, 2002). Zn is an anti-oxidant that reduces the production of reactive oxygen species (Bray and Bettger, 1990). Zn prevents lipids peroxidation and keeps the lysosomal membrane stable (Kimball et al., 1995). Zinc is a cofactor for Cu-Zn superoxide dismutase (Marklund et al., 1982). It plays an important role in catalase activity, regulating specificity protein 1 or other transcriptional response elements (Tate et al., 1995). Copper is required for animals as a trace element. It is intimately linked to hematopoiesis, metabolism and other vital life functions (Ognik et al., 2016). Feeding copper levels in the diet is essential for immune system optimization since copper inhibits the development of metabolic and oxidative stress in dairy cows (Cortinhas et al., 2010). It is also required for defense mechanisms, iron transport, cholesterol and glucose metabolism. Although copper isn’t an essential component of hemoglobin, it is found in several other plasma proteins which control iron release from cells into the plasma (Sloman et al., 2002). MATERIALS AND METHODS Experiment was approved by Institutional Animal Ethics Committee constituted as per the article number 13 of the 1 Department of Veterinary Physiology, College of Vaternity and Animal Sciences, Parbhani-431 402, Maharashtra, India. 2 Department of Animal Nutrition, College of Vaternity and Animal Sciences, Parbhani-431 402, Maharashtra, India. 3 Department of Poultry Science, College of Veterinary and Animal Sciences, Parbhani-431 402, Maharashtra, India. Corresponding Author: P.M. Kekan, Department of Veterinary Physiology, College of Vaternity and Animal Sciences, Parbhani- 431 402, Maharashtra, India. Email: drprakash73@gmail.com How to cite this article: Manimaran, S., Kekan, P.M., Daware, S.B., Wankar, A.K., Munde, V.K., Khose, K.K. and Bhagade, P.M. (2024). Effect of Copper and Zinc Supplementation on Antioxidants and Biochemical Status of Osmanabadi Goats. Indian Journal of Animal Research. 58(2): 293-297. doi: 10.18805/IJAR.B-4926. Submitted: 05-05-2022 Accepted: 03-08-2022 Online: 17-08-2022