1 Genetic code as an image of the mirror image. Supplement 2 Miloje M. Rakočević University of Niš, Faculty of Sciences and Mathematics, Department of Chemistry, Višegradska 33, 18000 Niš, Serbia Abstract In this (second) Supplement are given the further new insights on mirror symmetry within Genetic Code (GC). The main result is the correspondence of the binary tree of GC and the Standard GC Table in such a way that it follows that it makes no sense to talk about the evolution of GC from the aspect of test tube chemistry (of performing chemical reactions in nature and/or laboratory), but only makes sense to talk about prebiotic chemical evolution of GC in a manner analogous to the evolution of the chemical code i.e. Periodic system of chemical elements. Taken together, an even more important conclusion is that all the physical and chemical laws we know represent immediate causality, while in the case of GC on the scene is an indirect causality, realized through multiple mirror symmetry. Keywords: Protein amino acids, Genetic code, Binary tree, Golden mean, Periodic system, Mirror image. Mirroring. In Supplement 1 of this paper, we outlined the arrangement of amino acid pairs, in the order from the last to the first amino acid (AA) in the GC binary tree (Suppl. 1, Fig. 3 in relation to Fig. 1) 1 . In doing so, the first member of the pair was selected with strict adherence to two Mendeleevian principles - the principle of minimum change and the principle of continuity. [The only exception is the choice of asparagine rather than lysine because asparagine is a pair-member of aspartic acid, despite aspartic acid being previously paired with glutamic acid.] In the meantime, we have presented the distinction of amino acid molecules by the formula: [(6+3)+(3+4)+4]] 2 (Rakočević, 2020a), where subset {6+3} represents native AAs, subset {3+4} no-native AAs; both subsets within the set of {16} AAs of the alanine stereochemical type. The last number in summation is a set of {4} AAs of non-alanine stereochemical types. 3 Now it makes sense to combine the two distinctions (the order of 1 Figure 3 in Suppl. 1, here is Figure 1. Table 1 and Table 2 here contain the distribution of AAs in Table of standard GC. Table 3 shows, among other things, the pairs of AAs by chemical similarity. In relation to the pairing in Figure 1, here is the original chemical pairing: S-T and K-R but in Figure 1 the pairing is S-R and T-K. And, there is another pairing: here, in Table 3, the pairs are G-G (because glycine is the only one AA in the glycine stereochemical type; P-P (because proline is the only one in the proline type), V-I (because they are the only two AAs in the valine stereochemical type). In Figure 1, the pairs are G-V and P-I, as in CIPS 2 (Rakočević, 2019a; Fig. 1, p. 6). 2 It should be noted that number 4 is a neighbor, i.e. a follower of number 3 in the series of natural numbers (which is a condition for the validity of two Mendelian principles). We will hereafter refer to this formula as the Kinship Formula. Our prediction is that the Kinship Formula is a unique situation in a specific arithmetic system, which we will address in the next paper (Rakočević, 2020b). 3 On the four stereochemical types of AAs see in (Popov, 1989; Rakočević and Jokić, 1996).