Research Daacro & Saliva Lab, Trier, Germany; 6 Clinical Pharmacology Dpt, Inserm CIC 1405, CHU de Clermont-Ferrand, Universit e Clermont Auvergne, Clermont-Ferrand, France * Corresponding author. Rationale: Animal and clinical studies suggest complementary effects of Mg and vitB6 on stress. This is the first randomised trial to evaluate the effects of combined oral Mg and vitB6 on stress in a population with stress and low magnesemia using a validated measure of perceived stress. Methods: Phase IV, 8-week randomised, investigator-blind trial (EudraCT 2015-003749-24). Adults with a Depression Anxiety Stress Scales stress subscale score (DASS-42SS) >18 and serum Mg 0.5e0.85mmol/L (below mean value for healthy adults) were randomised 1:1 to oral Mg-vitB6 (Magne B6 ® , Mg 300mg; vitB6 30mg, QD) or oral Mg alone (Magnes- pasmyl ® , Mg 300mg, QD). Outcomes: change in DASS-42SS from baseline (BL) to Week (Wk)8 (primary) and Wk4; incidence of adverse events (AEs). Results: In the intention-to-treat analysis (n¼264), both treatments reduced DASS-42SS equally from BL to Wk8 (Mg-vitB6, 12.44 points 95% CI 13.83, 11.05; Mg, 11.72 points 95%CI 13.10, 10.33; p>0.05). An interaction (p¼0.0097) between BL stress level and treatment warranted subgroup analysis (per analysis plan); adults with BL severe/extremely severe stress (SESS) (DASS-42SS 25; n¼162) had 24% greater improve- ment with Mg-vitB6 vs Mg at Wk8 (3.16 points, 95%CI 0.50, 5.82; p¼0.0203); there was no difference between arms in the BL normal- emoderate stress subgroup (DASS-42SS <25; n¼102; p>0.05). Per protocol analysis and change from BL to Wk4 results were similar with greater improvement for Mg-vitB6 vs Mg in the SESS subgroup (ps<0.05). Fre- quency of AEs possibly related to treatment was similar in Mg-vitB6 (12.1%) and Mg-treated adults (17.4%). Conclusion: The addition of vitB6 to Mg was not superior to Mg alone to reduce stress in all adults with low magnesemia; superiority was demonstrated in severe/extremely severe stressed adults. Disclosure of Interest: E. Pouteau Other: Employee of Sanofi, M. Kabir- Ahmadi Other: Employee of Sanofi, L. Noah Other: Was an employee of Sanofi at the time of study conduct, A. Mazur Consultant for: Sanofi, un- related to this publication, L. Dye Grant / Research Support from: Sanofi, unrelated to this publication, Consultant for: Sanofi, unrelated to this publication, J. Hellhammer Consultant for: Sanofi, unrelated to this pub- lication, Other: Performance of saliva analyses for Sanofi, unrelated to this publication, G. Pickering: None declared, C. Dubray: None declared. SUN-LB410 INFLUENCES OF AGING ON EXPRESSION OF LIPOPOLYSACCHARIDE RECEPTOR OF KUPFFER CELLS IN MICE S. Murakoshi 1, * , K. Fukatsu 1 , M. Noguchi 1 , T. Watanabe 1, 2 , K. Higashizono 1, 3 , A. Watkins 1 , H. Yasuhara 1 . 1 Surgical Center, The University of Tokyo Hospital, Tokyo, Japan; 2 Department of Surgery, National Defense Medical College, Tokorozawa, Japan; 3 Department of Gastrointestinal Surgery, The University of Tokyo, Tokyo, Japan * Corresponding author. Rationale: The hepatic immunity generally declines with aging. Clinically, the consequences of impaired hepatic immune function in the elderly include an increased susceptibility to infections. In the previous study, phagocytic function of Kupffer cells (resident hepatic macrophages) de- clines with aging. However, the influence of aging on expression of lipo- polysaccharide (LPS) receptor’s expression of Kupffer cells is not clarified. Herein, we examined how the aging affects expression of LPS receptor (CD14) of Kupffer cells with mice model. Methods: We examined Kupffer cells of young adult (6 weeks old, n ¼ 7) and aged (65- to 70-weeks old, n ¼ 7) ICR mice. Whole liver was harvested and hepatic mononuclear cells (including Kupffer cells) were isolated. Hepatic mononuclear cells were incubated with or without LPS in vitro. CD14 expressions on Kupffer cells were evaluated in terms of mean fluo- rescence intensity (MFI) using flow cytometer. We also counted the number of Kupffer cells isolated. Results: Kupffer cells’ numbers were not significantly different between young adult and aged groups. Without LPS stimulation, there were no significant differences in the CD14 expressions on Kupffer cells between the 2 groups. However, with LPS stimulation, those of CD14 expressions were significantly less in the aged than in the young adult group (MFI of aged group ¼ 5.3 ± 0.2, MFI of young adult group ¼ 6.1 ± 0.3, P < 0.05, mean ± standard error, Mann-Whitney’s U test). Conclusion: Aging induced-reduction of LPS receptor’s expression of Kupffer cells might lead to impaired Kupffer cells’ function, and hepatic immunity in inflammatory condition. Disclosure of Interest: None declared. SUN-LB411 SERUM VISFATIN LEVEL AND ITS RELATIONSHIP TO ANTHROPOMETRIC AND METABOLIC PARAMETERS IN PREGNANCY INDUCED HYPERTENSION R. Nazli 1, * , A. Shaheen 1 , S. Fatima 1 , M.J. Khan 1 . 1 Biochemistry, Khyber Medical University Pakistan, Peshawar, Pakistan * Corresponding author. Rationale: Human adipose tissue is an important regulator of endocrine functions through its multisystem effects such as through the secretion of plasma adipocytokines. Out of these visfatin, deregulation participates in the pathogenesis of pregnancy induced hypertention. Methods: This was a cross sectional study conducted in three tertiary care hospitals of Peshawar. Serum visfatin levels (ng/mL) were determined by enzyme linked immune sorbent assay. Haematological parameters, liver function tests and serum electrolytes were determined by Sysmex hae- matology analyzer (Automated haematology analyzer). The blood urea and Lipid parameters were determined by automated chemistry analyzer in PGMI-LRH by using enzymatic kits of Roche diagnostics. BMI was calcu- lated using weight and height values. For data MINITAB® version 17 was used for further analysis Results: A strong positive and statistically significant association of visfatin was observed for monthly income (R 2 ¼7.75 and p-value <0.001). Similar result were obtained for still birth, cesarean section, low birth babies, family history of hypertension, systolic and diastolic BP, serum visfatin, serum albumin, serum ALP, serum chloride, serum HDL, serum LDL. Ratios of TC:HDL-C, LDL:HDL,TG:HDL and VLDL:HDL were having a positive and significant correlation. Conclusion: Serum visfatin was significantly associated with hematolog- ical parameters, liver function tests, serum electrolytes, and plasma lipid profile Disclosure of Interest: None declared. SUN-LB412 THE USE OF STANDARD PARENTERAL NUTRITION BAGS IN ADVANCED CANCER PATIENTS P.S. Patel 1, * , K.C. Fragkos 2 , N. Keane 1 , K. Murray 2 , S. Obbard 2 , S. Mehta 2 , F. Rahman 2 , S. Di Caro 2 . 1 Department of Nutrition and Dietetics, United Kingdom; 2 GI services, University College London Hospital, London, United Kingdom * Corresponding author. Rationale: The use of standard- compared to bespoke- PN bags may offer time and costs savings. Our aim is to compare HPN discharge scripts of advanced cancer patients with standard PN bags available on the market. Methods: Data was collected retrospectively for all patients with advanced cancer receiving HPN between 01.01.2016 and 15.10.2016 at University College London Hospital. Demographic variables at baseline and HPN scripts on discharge were collected. We conducted comparisons with 29 standard bags (SB) available on the UK market with each HPN discharge script by PN indication (bowel obstruction and high-output ostomy). Sta- tistical analysis included frequencies (%), means (SD). Results: 107 HPN patients with advanced cancer were included in this analysis (68 women, mean age 57yrs). The average daily HPN discharge script was: volume:2251 ± 626ml, nitrogen: 11 ± 3g, glucose:911 ± 304kcal, lipid:573 ± 263kcal, sodium:112 ± 61mmol, potassium:58 ± 26mmol, cal- cium:5 ± 2mmol, magnesium:10 ± 5mmol, and phosphate:21 ± 10mmol. Abstracts / Clinical Nutrition 37 (2018) S46eS314 S290