_____________________________________________________________________________________________________ *Corresponding author: E-mail: ebisonofongo@gmail.com; Asian J. Med. Prin. Clinic. Prac., vol. 7, no. 1, pp. 56-68, 2024 Asian Journal of Medical Principles and Clinical Practice Volume 7, Issue 1, Page 56-68, 2024; Article no.AJMPCP.112028 Phenotypic Characterization and in vitro Susceptibility Testing of Fungi Isolated from Madiga Bread (Local Staple Food) in Yenagoa Metropolis, Nigeria Oluwayemisi Agnes Olorode a and Ebipadei Zigabel Bowei-Ofongo a* a Department of Medical Laboratory Science, Niger Delta University, Amassoma, Bayelsa State, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Author OAO designed the study. Authors OAO and EZBO performed the experiments and analyzed the data. Authors EZBO and OAO drafted the manuscript. Both authors read and approved the final manuscript. Article Information Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/112028 Received: 24/11/2023 Accepted: 29/01/2024 Published: 07/02/2024 ABSTRACT Local bread known as "madiga" is typically enjoyed by residents of Nigeria's Niger Delta region. The aim of this study is to assess madiga bread for inbuilt pathogenic fungi in evaluating its level of edibility in Yenagoa Metropolis. A total of 42 samples was obtained and analyzed include; Hand swabs from factory workers (8/19%), hand swab of madiga vendors (6/14%), madiga bread gotten from market (12/29%), and madiga bread from factories (16/39%). The samples were processed using the following methods: culturing on Saboraud Dextrose Agar, identifying fungal isolates microscopic with Lacto-Phenol Cotton blue, staining the samples with Grama stain, germ tube test, Original Research Article