International Journal of Biochemistry, Biophysics & Molecular Biology 2019; 4(2): 25-30 http://www.sciencepublishinggroup.com/j/ijbbmb doi: 10.11648/j.ijbbmb.20190402.12 ISSN: 2575-5889 (Print); ISSN: 2575-5862 (Online) Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance Yulianela Mengana Torres 1, * , Manuel Arsenio Lores Guevara 1 , Juan Carlos García Naranjo 1 , Beatriz Taimy Ricardo Ferro 1 , Lidia Clara Suárez Beyries 2 , Inocente Clemente Rodríguez Reyes 2 , María A. Marichal Feliu 2 , Yomaidis Araujo Durán 2 1 Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba 2 Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba Email address: * Corresponding author To cite this article: Yulianela Mengana Torres, Manuel Arsenio Lores Guevara, Juan Carlos García Naranjo, Beatriz Taimy Ricardo Ferro, Lidia Clara Suárez Beyrio, Inocente Clemente Rodríguez Reyes, María Altagracia Marichal Feliu, Yomaidis Araujo Durán. Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. International Journal of Biochemistry, Biophysics & Molecular Biology. Vol. 4, No. 2, 2019, pp. 25-30. doi: 10.11648/j.ijbbmb.20190402.12 Received: December 11, 2018; Accepted: January 14, 2019; Published: January 10, 2020 Abstract: The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T 2 ). To experimentally determine the T 2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B 0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases. Keywords: Proton Magnetic Resonance, Dynamic Blood Plasma Viscosity and Hemoglobin 1. Introduction Hyperviscosity syndrome can occur associated with a variety of clinical diagnoses such as polycythemia, Waldenström's macroglobulinemia, multiple myeloma, leukemia, rheumatoid arthritis, asthma, sickle cell anemia and ICTUS [1-3]. This syndrome manifests in the form of bleeding, visual symptoms and neurological deficit that can reach the coma. The viscosity of the blood plasma and the hemoglobin solution can be determined with capillary, Falling body or rotational viscometers [4] and the method recommended by the International Committee for standardization in hematology is the Harkness viscometer, which is an automated capillary type [5]. However, these viscometers require at least 2 ml of sample, which can reach 10 ml or more, and they need to be washed after each determination, which is an invasive, cumbersome and time-consuming process. On the other hand, not all report the dynamic viscosity, being necessary to determine the density of the sample (ρ) by other methods (pignometry) in order to