Zahra Shamsi, Amir Atashi*, Saeid Abroun, Kaveh Tari, Mahshid AkhavanRahnama, Azadeh Anbarlou, Masoud Soleimani, Saeid Kaviani Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran * Corresponding author: Amir Atashi E-mail: atashia@modares.ac.ir Backgroundm: Induction of fetal hemoglobin (Hb-F) can improve the patients’ symptoms of haemoglobinopathies. Several factors can induce gamma globin gene expression and increased Hb-F levels in patients. In this sudy, the expression of genes is involved in regulation of gamma globin synthesis such as PIPKII-alpha, BCL11a, and miR- 30a during CD34+ hematopoietic sem cell diferentiation into erythroid lineage was sudied. Material and Methods: In this sudy, CD34+ cells were isolated from umbilical cord blood. Then, CD34+ cells were cultured in diferentiation medium. Erythroid colonies were harvesed on days 8, 11, 14 and gene expression of gamma globin, PIPKII-alpha, BCL11a, and miR-30a were analyzed by quantitative RT- PCR. Results: : The results showed that gene expression of gamma-globin gradually at the 8th day after diferentiation sarted and at 14th day reached to the highes level of its expression. In parallel with increasing the expression of gamma-globin (22-folds), the expression of PIPKII-alpha increased (2.5-folds), while the expression of BCL11a decreased (1.2-folds). Moreover, the expression of miR- 30a increased during diferentiation into erythroid lineage (2.3-folds) (P<0.05). Conclusion: The results indicated that increased expression of PIPKII-alpha and decreased expression of BCL11a during CD34+ cells diferentiation to erythroid lineage. According to expression pattern of miR-30a and its target genes, PIPKII-alpha and BCL11a, we cannot suppose the inhibitory efect of miR-30a on PIPKII-alpha expression, but due to inhibitory efect of miR-30a on BCL11a gene, we can introduce this microRNA as a regulatory molecule in the induction of Hb-F pathway. However, the further sudies are needed to invesigate this interaction. Keywords: Hemoglobinopathy, Gamma globin, PIPKII-alpha, BCL11a, miR-30a Absract Research on Medicine Original Article The Quarterly journal of School of Medicine, Shahid Beheshti University of Medical Sciences (Received: 2016/04/14 Accept:: 2016/11/14) Vol.40; No.3; 2016 Research in Medicine 2016; Vol.40; No.3; 125-130 The evaluation of expression of genes, which are involved in gamma globin synthesis before and after diferentiation of hematopoietic sem cells into erythroid lineage Downloaded from pejouhesh.sbmu.ac.ir at 9:13 +0330 on Sunday December 5th 2021