Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2013, Article ID 538673, 5 pages http://dx.doi.org/10.1155/2013/538673 Research Article The Drinking Effect of Hydrogen Water on Atopic Dermatitis Induced by Dermatophagoides farinae Allergen in NC/Nga Mice Rosa Mistica C. Ignacio, 1 Hyun-Suk Kwak, 2 Young-Uk Yun, 2 Ma. Easter Joy V. Sajo, 1 Yang-Suk Yoon, 1 Cheol-Su Kim, 3 Soo-Ki Kim, 3 and Kyu-Jae Lee 1 1 Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 220-701, Republic of Korea 2 ECO Solution Team, Digital Media and Communications R&D Center, Samsung Electronics Co., Ltd., Suwon, Gyeonggi 443-742, Republic of Korea 3 Department of Microbiology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 220-701, Republic of Korea Correspondence should be addressed to Kyu-Jae Lee; medbio9@gmail.com Received 13 September 2013; Revised 28 October 2013; Accepted 5 November 2013 Academic Editor: K. B. Harikumar Copyright © 2013 Rosa Mistica C. Ignacio et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Hydrogen water (HW) produced by electrolysis of water has characteristics of extremely low oxidation-reduction potential (ORP) value and high dissolved hydrogen (DH). It has been proved to have various benefcial efects including antioxidant and anti- infammatory efects; however, HW efect on atopic dermatitis (AD), an infammatory skin disorder, is poorly documented. In the present study, we examined the immunological efect of drinking HW on Dermatophagoides farinae-induced AD-like skin in NC/Nga mice. Mice were administered with HW and purifed water (PW) for 25 days. We evaluated the serum concentration of pro-infammatory (TNF-), T1 (IFN-, IL-2, and IL-12p70), T2 (IL-4, IL-5, and IL-10), and cytokine expressed by both subsets (GM-CSF) to assess their possible relationship to the severity of AD. Te serum levels of cytokines such as IL-10, TNF-, IL-12p70, and GM-CSF of mice administered with HW was signifcantly reduced as compared to PW group. Te results suggest that HW afects allergic contact dermatitis through modulation of T1 and T2 responses in NC/Nga mice. Tis is the frst note on the drinking efect of HW on AD, clinically implying a promising potential remedy for treatment of AD. 1. Introduction Atopic dermatitis (AD) is an allergic infammatory skin disorder characterized by impaired immunological responses [1]. AD usually afects 10% to 20% infants and young children, but it can persist into adulthood (1%–3%) since it is ofen a long-lasting skin disease [2]. AD has a complex etiology involving interrelationship of several factors such as genetic, environmental, pharmacologic, psychological, immunologic and skin barrier dysfunction [3]. Te altered immune func- tion received special attention as the major factor contribut- ing to the onset, development, and severity of AD. AD is well characterized by having clinical phenotype such as elevated serum IgE levels, peripheral eosinophilia, and eczematous skin lesions infltrated by infammatory cells [4, 5]. NC/Nga mice were the frst representative animal model for inves- tigating and developing treatment on AD-like skin disease [6, 7]. In conventional surroundings, NC/Nga mice were observed to spontaneously develop skin lesions characterized by scratching behavior, erythema and hemorrhage, edema, scaling, and dryness of the skin comparable to human AD [6]. Under pathogenic-free surrounding, NC/Nga mouse model does not show skin lesions and thus AD-like symptoms are triggered by exposure to a stimulus. Dermatophagoides farinae (Df) is a cosmopolitan species of house dust mites and a common contributory cause of AD. Te topical application of this allergen Df extract (DfE) produces atopic dermatitis- like skin lesions in NC/Nga mice [8, 9]. Terefore, we adopted