ABSTRACT The reaction of 2-amino-benzothiazole with bis [O,O-2,3,O,O – 5,6 – (chloro(carboxylic) methiylidene) ] – L – ascorbic acid (L-AsCl2) gave new product 3-(Benzo[d]Thaizole-2-Yl) – 9-Oxo-6,7,7a,9-Tertrahydro-2H-2,10:4,7-Diepoxyfuro [3,2-f][1,5,3] Dioxazonine – 2,4 (3H) – Dicarboxylic Acid, Hydro-chloride (L-as-am)), which has been insulated and identifed by (C, H, N) elemental microanalysis (Ft-IR),(U.v–vis), mass spectroscopy and H-NMR techniques. The (L-as am) ligand complexes were obtained by the reaction of (L-as-am) with [M(II) = Co,Ni,Cu, and Zn] metal ions. The synthesized complexes are characterized by Uv–Visible (Ft –IR), mass spectroscopy molar ratio, molar conductivity, and Magnetic susceptibility techniques. (L-as-am) ligand analysis exhibited that metal ions coordinated with (L-as-am) thought the bidentate carboxyylate, sulfur atom from one side and the other side with bidentate carboxylate and nitrogen atom forming binuclear six - coordinated metal ions in an octahedral geometry. The purity of (L-as-am) ligand and their complexes were screened using Thin-layer chromatography (TLC). Also, the study of the biological activity has been conducted for (L-as-am) ligand and its complexes, which showed diversity in its activity towards two types of pathogenic bacteria, (Escherichia coli, Staphylococcus aureus) and also against (Candida albicans) fungi. Keywords: Synthesis, (L-as-am), Characterized, Metal Complexes, Biological activity. International Journal of Drug Delivery Technology (2021); DOI: 10.25258/ijddt.11.3.00 How to cite this article: Badeea YA , Mahdi WK, Ibrahim Y, Musa FH. Synthesis, Characterization Of Some Metal Complexes With 3-(Benzo[d]Thaizole-2-Yl)-9-Oxo-6,7,7a,9-Tertrahydro-2H-2,10:4,7-Diepoxyfuro [3,2-f][1,5,3] Dioxazonine–2,4 (3H)– Dicarboxylic Acid. Hydro-chloride (L–as–am) and Study their Biological Activity. International Journal of Drug Delivery Technology. 2021;11(3):1-7. Source of support: Nil. Confict of interest: None Synthesis, Characterization Of Some Metal Complexes With 3-(Benzo[d] Thaizole-2-Yl)-9-Oxo-6,7,7a,9-Tertrahydro-2H-2,10:4,7-Diepoxyfuro [3,2-f][1,5,3] Dioxazonine–2,4 (3H)–Dicarboxylic Acid. Hydro-chloride (L–as–am) and Study their Biological Activity Yasamen A. Badeea, 1 Waleed K. Mahdi, 1 Yousif Ibrahim, 1 Falih H. Musa 2* 1 Department of Chemistry, College of Education of pure science, Ibn – Al Haitham, University of Baghdad, Baghdad, Iraq 2 Head of Medical Laboratory Techniques Department, Ashur University, Collage Universty, Baghdad, Iraq Received: 2nd June, 2021; Revised: 12th July, 2021; Accepted: 30th July, 2021; Available Online: 25th September, 2021 INTRODUCTION Ascorbic acid is the important source for the synthesis of many chemical compounds, representing the basic base used to prepare its derivatives required in the synthesis of coordination complexes. In terms of competence, collagen synthesis has various physiological and pharmacological functions, 1,2 anti-oxidation, 3,4 and antibacterial activity. 5 Another derivative of L-ascobic acid has been synthesized, such as 5,6-O-isopropylidene-2,3-(2-X,1-carboxyl) deoxy-L-ascorbic acid, where X = I, Cl, 1,2-dihydroxyethyl-1-(2-mercaptophenyl)- 5-(2-mercaptophenyl)-2,5-dihydro – 1H – pyrol – 3,4 – diol, 5,6 – O,2, 3 – O – L-ascorbic acid were substituted by acetic acid. The thiol derivative of bis [ O, O – 2,3,O, O – 5,6 –(carboxylic) methylidene) ] – L-ascorbic acid has been prepared. 5,6 L – ascorbic derivatives of metal ions complexes were also synthesized and characterized, and their biological efect has RESEARCH ARTICLE been noticed. 5-7 The research aims to synthesize new derivatives for L-ascorbic acid of (L-as-am) ligand with metal (II) ions complexes when, M (II) = (Zn, Cu, Ni, Co). MATERIALS AND METHODS Experimental Chemicals Metal salts (CoCl 2 . 6H 2 O, NiCl 2 . 6H 2 O, CuCl 2 . 2H 2 O, ZnCl 2 . 2H 2 O) have been acquired from fuka, Merck, L–ascorbic acid, 2-aminobenzothiazol (fuka) Tri chloro acetic acid (Riedel-de Haën). Instrumentations 1. The electronic spectra were measured on (UV-Vis) spectroscopy kind Shimadzu UV-160A at the wavelength range (200–1000) nm, using match quartz cells (1 cm) and water as a solvent at service laboratory in Chemistry Department, College of Education of Pure Science, Ibn *Author for Correspondence:Falil H. Musa; E-mail:falih.hassan@au.edu.iq