Int J Cur Res Rev | Vol 12 • Issue 22 • November 2020 S-3 Corresponding Author: Dr. M. A. Ehtesham, Department of Paediatrics, Datta Meghe Institute of Medical Sciences, Sawangi, Wardha, India. Contact: 7013562560; Email: drehty@gmail.com ISSN: 2231-2196 (Print) ISSN: 0975-5241 (Online) Received: 20.08.2020 Revised: 15.09.2020 Accepted: 25.10.2020 Published: 30.11.2020 International Journal of Current Research and Review DOI: http://dx.doi.org/10.31782/IJCRR.2020.SP84 INTRODUCTION Pyruvate to lactate in a glycolytic pathway is caused by en- zyme lactate dehydrogenase (LDH) along with the conver- sion of nicotinamide adenine dinucleotide to nicotinamide adenine dinucleotide +. Lactate dehydrogenase can be found in all the body tissues. Lactate dehydrogenase is found in five different isoenzymes types 1-5 as by their electropho- retic motility. 1 Their distribution of all the types of LDH is different across different body tissues. 1, 2 can be found in RBC and heart smooth muscle; type 3 is found to be highest in lungs; type 4 is found to be highest in the kidney, placenta, pancreas; and type 5 is found in increased levels in skeletal muscle and liver. 2 The serum LDH routine evaluation in- cludes all of the 5 types. Along with increase reticulocyte count, increase in indi- rect bilirubin level, and decrease in serum haptoglobin, Lactate dehydrogenase (LDH) also has been used as a marker of hemolysis. In sickle cell anaemia in the steady- state (SS) there will be an increase in the level of lactate dehydrogenase(LDH). 3 And when painful vasoocclusive crises (VOCs) like dactylitis, sickle cell hepatopathy and abdominal crisis, and acute chest syndrome, the lactate de- hydrogenase levels may further increase in some sickle cell patients due to increase in hemolysis 4 . In one study Neely et al found that increase in serum LDH was not correlated with plasma Hb level, indicating that the source of LDH is not secondary to hemolysis but rather to tissue damage. 1 In a forthcoming enlightening partner concentrate in young- sters between a half year to 18 years indicated that the LDH chemical level increments fundamentally during an emer- gency contrasted and consistent state esteems and that there is a critical positive relationship between’s LDH levels and the seriousness of pain. 5 however, raised LDH levels during . ABSTRACT Introduction: Pyruvate to lactate in a glycolytic pathway is caused by enzyme lactate dehydrogenase (LDH) with the conver- sion of NADH TO NAD+. Lactate dehydrogenase can be found in different body tissues. For hemolysis marker lactate dehydro- genase levels can be used. In sickle cell anaemia patient those who are in the steady-state (SS) there will be an increase in the level of lactate dehydrogenase(LDH). In sickle cell disease, the life of the patient is hampered due to crisis and it is very difficult to predict the crisis. Hence in our study, we used a marker lactate dehydrogenase (LDH ) to predict the severity of the crisis. Objectives: To study the ability of serum LDH levels in predicting the severity of disease in sickle cell anaemia. Methodology: To achieve the objective we assess serum LDH levels during steady-state and crisis and to correlate above with chronic complications (PH, Microalbuminuria, Liver functions) and severity of the crisis.Patients fulfilling selection criteria will be recruited after obtaining written consent. Detailed history including the age of onset, frequency and severity of the crisis, frequency of admissions and transfusions, quality of follow up, quality of treatment received will be taken. Detailed examination including anthropometry will be recorded. The severity of the acute crisis will be decided based on the duration of pain more than 2 days, need for admission & stay more than 3 days, death, need for surgery, need for support and need for other procedure. Expected Results: We expect the LDH level to be significantly higher during the crisis. If steady-state patients are considered LDH level will be high in patients with moderate and severe disease (frequency more than 3/ year). Key Words: Lactate Dehydrogenase, Sickle Cell Disease, Prediction Lactate Dehydrogenase as Predictor for Severity in Sickle Cell Disease M. A. Ehtesham 1 , Bhavana Lakhkar 2 , Rakesh Adkane 3 1 Resident, Department of Paediatrics, Datta Meghe Institute of Medical Sciences, Sawangi, Wardha, India; 2 Professor and Head Department of Paediatrics, Datta Meghe Institute of Medical Sciences, Sawangi, Wardha, India; 3 Professor, Mechanical Engineering, Yeshwantrao Chavan College of Engineering, Nagpur, India. IJCRR Section: Healthcare Sci. Journal Impact Factor: 6.1 (2018) ICV: 90.90 (2018) Copyright@IJCRR Study Protocol