How to Cite: Tassew, M. F., & Chouhan, G. (2022). Biosynthesis and antibacterial activity of Passiflora incarnata mediated copper oxide nanoparticles. International Journal of Health Sciences, 6(S4), 6059–6075. https://doi.org/10.53730/ijhs.v6nS4.9529 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 27 March 2022, Manuscript revised: 9 May 2022, Accepted for publication: 18 June 2022 6059 Biosynthesis and antibacterial activity of Passiflora incarnata mediated copper oxide nanoparticles Molla Fentie Tassew Department of Biotechnology, School of Engineering and Technology, Sharda University, Knowledge Park III, Greater Noida, Uttar Pradesh, India Garima Chouhan Department of Biotechnology, School of Engineering and Technology, Sharda University, Knowledge Park III, Greater Noida, Uttar Pradesh, India Corresponding author email: garimachouhan68@gmail.com Abstract---Plant mediated nanoparticle synthesis is a rapidly growing field of study around the world. Plant biomolecules make non-toxic, stable, and cost-effective copper oxide nanoparticles In this study, eco-friendly CuONPs were prepared through a biosynthetic approach using Passiflora incarnata L. leaf extract. The synthesized CuONPs were characterized using UV-Visible spectroscopy (UV-Vis), scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Dynamic light scattering (DLS). Furthermore, the antibacterial activity was evaluated against Listeria monocytogenes. The SEM results indicated that rod-shaped CuONPs with an average particle size of 49.8 nm (size range of 39-63 nm) were synthesized and shown good antibacterial activity against Listeria monocytogenes. CuONPs produced from Passiflora incarnata leaf extract could be a potential antibacterial agent for treating foodborne infections caused by Listeria monocytogenes. However, more research is needed to determine how it works on the target microorganism. Keywords---passiflora incarnata, biosynthesis, antibacterial activity, listeria monocytogenes. Introduction Copper oxide nanoparticles (CuONPs) have gotten attentions because of their unique chemical and physical features compared to other nanoparticles(Rathnakumar et al., 2019; Sau & Rogach, 2010). In comparison to other metals such as platinum, silver, and gold, copper is commonly utilized in