ISSN: 2320-5407 Int. J. Adv. Res. 6(2), 1909-1916 1909 Journal Homepage: -www.journalijar.com Article DOI:10.21474/IJAR01/6591 DOI URL: http://dx.doi.org/10.21474/IJAR01/6591 RESEARCH ARTICLE ANALYSIS OF MICROBIOLOGICAL CONTAMINATION IN AQUIFERS, IN THE URBAN REGION OF OBLIGADO AND HOHENAU – PARAGUAY. Alicia Raquel Eisenkölbl Closs 1 , Estelvina Rodríguez Portillo 2 , Rafaela Laino 3 and Antonio Aníbal Benítez Cañiza 4 . 1. Postgraduate School, National University of Itapúa, Encarnación, Paraguay. 2. School of Science and Technology, National University of Itapúa, Encarnación, Paraguay. 3. Chaco Americano Research Center, Manuel Gondra Foundation. 4. Catholic University – School of Agricultural Sciences. …………………………………………………………………………………………………….... Manuscript Info Abstract ……………………. ……………………………………………………………… Manuscript History Received: 25 December 2017 Final Accepted: 27 January 2018 Published: February 2018 Keywords:- Drinking Water, Water Quality, Total Coliforms, Underground Water, Water Supply In Paraguay, 85% of homes have water supply from enhanced sources, 63% are delivered by a distribution system. Likewise, 79% of homes have sanitary drains and 11% are connected to an effluent treatment system (Secretaría Técnica de Planificación del Desarrollo Económico y Social, 2015), this condition hints to superficial pollution problems as well as underground water. The Capiibary Stream watershed, situated in the Department of Itapúa, Paraguay and established on the Guarani Aquifer System (SAG, in Spanish), aquifers exploited by deep wells where the presence of fecal coliform is found in 60% of sampled wells (Protección y Manejo de Aguas Subterráneas en Paraguay PAS-PY, 2012). The study calculated variations in total coliforms in groundwater watershed detected in the center of Obligado and Hohenau, the analysis of groundwater property was based on data from 59 water samples taken over a 10-year period (2006-2016) in 17 deep wells, variations of total coliforms were estimated. For the statistical processing, the analysis of variance and contrast of means (Duncan test) with margin of error 5% were performed. It was determined the variations of the overall coliforms in the study period 2006 to 2016, the presence of total coliforms topped the maximum acceptable threshold. Similarly, deep wells were detected in Obligado, whose standards extended up to 998 CFU / 100ml. Lastly, the results were contrasted with World Health Organization (WHO, 2006) parameters and the Official Paraguayan Standards ERSSAN (ERSSAN, 2017) Law Nº. 1614/2000. Copy Right, IJAR, 2018,. All rights reserved. …………………………………………………………………………………………………….... Introduction:- Global groundwater extraction increased from 312 km 3 /year in the 1960s to 743 km 3 /year in 2000. Nearly 70% of the water extracted is used for farming, the remaining 30% corresponds to human consumption. Also, half of drinking water in urban regions comes from groundwater (Wada et al., 2010). Corresponding Author:- Alicia Raquel Eisenkölbl Closs. Address:- Postgraduate School, National University of Itapúa, Encarnación, Paraguay.