ACTA SCIENTIFIC Medical SCIENCES (Issn: 2582-0931) Volume 4 Issue 1 January 2020 Spectroscopic Study on Tubacam nicotina Leaves and Commercial Tobacco in the UV, IR Regions and Its Phytochemical Screening of Natural Native Tobacco from Libya Fatimh Meelad 1 , Salem Edrah 2 *, Hanan Alnade 3 , Adel Mlitan 4 and Huda Elgubi 4 1 Chemistry Department, Faculty of Education, El- Mergib University, Khums Libya 2 Chemistry Department, Faculty of Science, El- Mergib University, Khums Libya 3 Chemistry Department, Faculty of Science, Alasmarya Islamic University, Zliten Libya 4 Chemistry Department, Science College, Misurata University, Misurata Libya *Corresponding Author: Salem Edrah, Chemistry Department, Faculty of Science, El- Mergib University, Khums Libya. Research Article Received: November 11, 2019; Published: December 11, 2019 Abstract The leaves extracts of natural and commercial used tobacco sample tested on UV, IR spectrum and phytochemical screening. The colour of the natural tobacco extract was dark green, it’s shape was mucilaginous and has pH 8.35, while the colour of the commercial tobacco extract was dark brown, it’s shape was mucilaginous and has pH = 7.6. Furthermore, percentage yield of the raw extract of tobacco leaves plant and the raw extract of commercial tobacco were 12.723 and 7.341%, respectively. While percentage yield of alkaloids for tobacco leaves and alkaloids for commercial, tobacco was 6.32 and 4.13% correspondingly. The results of the qualitative detection tests revealed the presence of alkaloids in natural tobacco and commercial tobacco extracts. The UV spectrum of nicotine from Tubacam nicotina corresponds to the wavelength of the maximum absorption peak (λ Max of the compound) with the radiation spectrum of the standard compound also. Although the UV spectrum of nicotine form, commercial tobacco corresponds to the wavelength of the maximum absorption peak (λ Max of the compound) with the radiation spectrum of the standard compound. Furthermore, the absorption of nicotine in the infrared region, revealed to be identical to the IR absorption of the Nicotine standard, at 2917 cm where the vibration frequencies of the most critically active groups in the compound. Keywords: Tubacam nicotina, Commercial Tobacco, UV, IR spectroscopy, Phytochemical Screening Tobacco is the common name for several plants of the Nicotiana genus and belongs to the Solanaceae (nightshade) family. Tobacco is a plant of about 2 meters tall with red flowers and large green leaves and owned about 60 different species. The most common plant species are tobacco smoke (Tubacam nicotina) [1,2]. Nicotine is a stimulant and potent Para sympathomimetic alkaloid that is naturally produced in the nightshade family of plants, and is used as a treatment of addictions and ailments caused by tobacco misuse such as a smoking addiction where the substance can help relieve the recipient from withdrawal symptoms [3- Introduction 5]. Likewise nicotine plays a very important rule in nicotinic acetylcholine receptors (nAChRs) [6-8], Correspondingly nicotine constitutes of 0.6–3.0% of the dry weight of tobacco [9], and usually compatible concentrations of nicotine varying from 2–7 µg/kg (20–70 millionths of a per cent wet weight), are found in the edible family Solanaceae such as potatoes, tomatoes, and eggplant [10]. Some studies indicate that the contribution of nicotine obtained from food is substantial in comparison to the inhalation of second-hand smoke [10]. Where others consider nicotine obtained from food to be trivial unless exceedingly high amounts of certain vegetables are eaten [10]. It functions as an DOI: 10.31080/ASMS.2020.04.0499 Citation: Salem Edrah., et al. “Spectroscopic Study on Tubacam nicotina Leaves and Commercial Tobacco in the UV, IR Regions and Its Phytochemical Screening of Natural Native Tobacco from Libya”. Acta Scientific Medical Sciences 4.1 (2020): 69-75.