78 Protein Spesifik Cairan Kista Cysticercus bovis pada Sapi Bali yang Diinfeksi dengan Taenia saginata (SPECIFIC PROTEIN OF CYSTICERCUS BOVIS CYST FLUID ON BALI CATTLE EXPERIMENTALLY INFECTED WITH TAENIA SAGINATA) Nyoman Sadra Dharmawan 1, 4 , I Made Dwinata 1, 2 , Kadek Swastika 2 , I Made Damriyasa 1, 4 , Ida Bagus Made Oka 1, 2 , I Nyoman Mantik Astawa 3 1 Grup Riset Center for Studies on Animal Disease, 2 Laboratorium Parasitologi, 3 Laboratorium Virologi, 4 Laboratorium Patologi Klinik Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali Tlp. 0361223791. E-mail: nsdharmawan@yahoo.com ABSTRAK Cysticercus bovis adalah fase larva dari cacing pita daging sapi (Taenia saginata). Infeksi larva ini pada sapi disebut Bovine cysticercosis atau Cysticercosis bovis. Bovine cysticercosis ditemukan di seluruh dunia, terutama di negara-negara yang sedang berkembang, di wilayah dengan kondisi tidak higenis, manajemen peternakan buruk, dan tidak dilakukannya pemeriksaan kesehatan daging. Infeksi cacing dewasa Taenia pada manusia disebut taeniasis. Taeniasis Taenia saginata juga ditemukan hampir di seluruh dunia. Di Kabupaten Gianyar Bali, prevalensinya dilaporkan mencapai 27,5%. Untuk pengendalian, vaksinasi terhadap C. bovis memainkan peran penting di daerah endemis. Penelitian ini bertujuan untuk menentukan protein dalam cairan kista C. bovis yang bersifat imunogenik yang dapat dikembangkan menjadi kandidat vaksin. Lima ekor mencit diimunisasi empat kali dengan cairan kista dan serum yang diperoleh dari mencit dipakai dalam uji Western Blotting. Untuk melihat protein C. bovis dalam cairan kista, dilakukan elektroforesis menggunakan sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) dan diwarnai dengan pengecatan C. blue. Untuk menentukan protein imunogenik, protein ditransfer ke membran nitroselulosa dan direaksikan dengan serum mencit yang diimunisasi dengan cairan kista C. bovis. Hasil penelitian menunjukkan bahwa dengan SDS-PAGE ditemukan sekitar 17 protein dengan berat molekul antara 14,86 kDa sampai 122,40 kDa. Sementara itu dengan uji Western Blotting ditemukan tujuh protein yang imunogenik dengan bobot molekul: 16,81 kDa; 19,22 kDa; 20,98 kDa; 27,41 kDa; 34,02 kDa; 38,31 kDa; dan 54,94kDa. Kata kunci: imunogenik, cairan kista dari Cysticercus bovis. ABSTRACT Cysticercus bovis is the larval stage of Taenia saginata, the bovine tapeworm. The infection of this larval in cattle musculature causes Bovine cysticercosis or Cysticercosis bovis. Bovine cysticercosis is found worldwide, but mostly in developing countries, where unhygienic conditions, poor cattle management practices, and the absence of meat inspection are common. The adult Taenia infection in man is referred to as taeniasis. Taenia saginata taeniasis is also found almost all over the world. The prevalence of Taenia saginata taeniasis has reported up to 27.5% in Gianyar Bali. In order to control the diseases, vaccination against the larvae stages in cattle of Taenia saginata may play an important role in controlling the disease in the endemic regions. The aims of the present study were to prepare and to investigate the immunogenic protein as vaccine candidate for controlling Cysticercus bovis infection in in Bali cattle. Cysticercus protein from the cyst fluid was firstly used to immunize mice and the mice sera were then collected. Cysticercus proteins then analyzed using sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE). All cysticercus proteins were then visualized by Commasie blue staining. The proteins were also transferred onto nitrocellulose membrane and the immunogenic proteins were visualized by Western Blotting using immune sera raised in mice. By Commasie blue staining, a total of 17 proteins were detected with the molecular weight of 14,86 kDa -122,40 kDa from the smallest to the largest. As many as 7 immunogenic proteins with the molecular weights of 16.81 kDa; 19.22 kDa; 20.98 kDa; 27.41 kDa; 34.02 kDa; 38.31 kDa; and 54.94kDa were detected. Keywords: immunogenic, cyst fluid of Cysticercus bovis. Jurnal Veteriner Maret 2013 Vol. 14 No. 1: 78-84 ISSN : 1411 - 8327