167 on the SAT reading comprehension exam, but only following EX (r= -0.59, P=0.025). Following QR, SA after the first set of cognitive tests was associated with worse SAT reading comprehension (r= -0.53) and math scores (r= -0.598); this effect was not seen following EX and was seen even after controlling for baseline SA [r(12)= -0.64, P= 0.014]. CONCLUSIONS: Consistent with previous research, SA was reduced following both EX and QR. Additionally, exercise has been shown to buffer SA increases following a stressor. In the present study, EX buffered the anxiogenic response that accompanied cognitive testing. This extends previous literature to include individuals with high test anxiety, and provides evidence that EX has a beneficial effect on academic performance in such individuals. In addition to its physical health benefits, EX has application as a coping mechanism for exam taking, if not for completely preventing or dampening anxiety reactions. This research was supported by the Raymond and Rosalee Weiss Research Endowment from the American College of Sports Medicine Foundation 653 Board #49 May 27, 2:00 PM - 3:30 PM Physical Activity And Eating Habits In A Young Population. Cristian Petri 1 , Gabriele Mascherini 1 , Vittorio Bini 2 , Nicola Armentano 1 , Pier Giuseppe Calà 1 , Giorgio Galanti 1 . 1 Sport Medicine Department, Florence, Italy. 2 University of Perugia, Perugia, Italy. Email: gabriele.mascherini@unifi.it (No relationships reported) PURPOSE: Health-related behaviors such as physical activity and eating habits have been recognized as key aspects of lifestyle. Lifestyle habits adopted in adolescence can be traced into adulthood. These influence the risk of not communicable chronic disease. The aim of the study was to evaluate the physical activity (PA) and eating habits (EH) in young people. METHODS: 922 young people - 719 males (M 78.0%) and 203 females (F 19.8%) - ages 8 to 18 (age 13.7±2.7 yr, weight 54.6±15.2 kg, height 163.3±15.5 cm) were enrolled. During the evaluation for sport eligibility in the Sports Medicine Department, we asked participants to complete a questionnaire in order to assess physical activity and eating habits (INRAN, Italian Institute for Research in Food and Nutrition). THE QUESTIONNAIRE INVESTIGATED: - PA: <1h in a week as not adequate, > 1h in a week as adequate; - EH: weekly frequencies of food groups, food allergies and frequency and type of supplements (S), site where meals were consumed. Statistical analyses were performed using a two-sided p-value <0.05 which was considered significant. RESULTS: Normal weight (NW): 772 subjects (86.3 %); Overweight (OW) 111 subjects (12.4 %, 80 M, 31 F); Obese (OB) 12 subjects (1.3%, 8 M, 4 F). 473 NW (62.3 %) are PA adequate; 286 (37.7%) PA are not adequate. 61 OW (55.5 %) are PA adequate and 49 (44.5%) are not adequate. 5 OB (41.7%) are PA adequate while 7 (58.3%) reported no PA in addition to sports practiced. Those who have more PA show less BMI (p=0.001), NW subjects have more PA during a week (p=0.03) in comparison to OW subjects. EH of whole sample are incorrect; in addition OW children eat less cereals (p=0.02), dried fruits (p=0.02) and desserts (p=0.006) in comparison with the international guidelines. 14.7% have reported taking S and 13% of these habitually. The S predominantly taken were Vitamins + Minerals. CONCLUSIONS: This study observes a population who does not follow international lifestyle guidelines. Eating habits are incorrect and the global physical activity is low respect as recommended: despite they regularly do sports activity, 13.7% are OW and OB. The goal of this intervention is to avoid the increase in body mass in young people, and therefore to prevent non communicable chronic disease in adulthood. Higher education is necessary in order to promote a healthy lifestyle in terms of EH and PA. 654 Board #50 May 27, 2:00 PM - 3:30 PM The Seated Inactivity Trial (S.I.T): Health Outcomes Associated with Eight Weeks of Imposed Sedentary Behavior Brooke J. Cull, Richard R. Rosenkranz, FACSM, Mark D. Haub, Sara K. Rosenkranz. Kansas State University, Manhattan, KS. (Sponsor: Richard R. Rosenkranz, FACSM) Email: brooke1@ksu.edu (No relationships reported) Sedentary time, independent of physical activity levels, is a risk factor for several chronic diseases and all-cause mortality. The negative effects of extreme amounts of sedentary behavior have been examined (e.g., bed rest and detraining studies in fit individuals); however, more true-to-life trials are necessary to understand the health implications of increased sedentary time. PURPOSE: The objective of this study was to determine whether imposing ten hours of sedentary time per week for eight weeks would alter the cardiometabolic risk profile of physically active adults. METHODS: Sixteen healthy young adults (aged 21.6 ± 1.4 years, 10 males) who met physical activity guidelines (>150 minutes moderate-to-vigorous physical activity/week) were randomized to either the sitting (SIT) group or no-intervention control (CON) group. SIT participants attended monitored sedentary sessions for eight weeks (10 hrs/week). Laboratory assessments at baseline, week four, and week eight included: blood pressure; body composition (DEXA scan); fasting blood samples; physical activity levels (seven days of accelerometry, via Actical at wrist); and three-day food records. RESULTS: SIT and CON participants were not different at baseline for cardiometabolic risk factors (p>0.05). Systolic blood pressure increased in SIT, but not CON, from baseline to week four (SIT: 10.1 ± 7.2 mmHg; CON: -7.3 ± 9.6 mmHg; p=0.001), and remained elevated at week eight. At week eight, there was a significant increase in total cholesterol from baseline for SIT (11.1 ± 12.5 mg/dL, p<0.05), but not CON (1.1 ± 22.2 mg/dL, p>0.05), with no between-group differences. The change in systolic blood pressure was associated with a change in total cholesterol (r=0.51, p=0.04). There were no changes in fasting glucose or body composition. Physical activity did not change, but SIT participants decreased caloric intake from baseline to week eight (p<0.05). CONCLUSION: Following eight weeks of imposed sedentary time, physically active adults increased systolic blood pressure and total cholesterol, but did not exhibit changes in other risk factors. Imposing just 10 hours per week of sedentary behavior appeared to have an adverse impact on cardiometabolic health in physically active adults. 655 Board #51 May 27, 2:00 PM - 3:30 PM Moderate Exercise Does Not Acutely Attenuate Cardiometabolic Function Associated with “Sitting Disease” Amanda Younger 1 , Robert Pettitt 1 , Patrick Sexton 1 , William Maass 1 , Thomas W. Kernozek, FACSM 2 , Cherie Pettitt 1 . 1 MNSU, Mankato, Mankato, MN. 2 University of Wisconsin-LaCrosse, LaCrosse, WI. (No relationships reported) Viola Holbrook Human Performance Laboratory, Minnesota State University, Mankato, Mankato, MN, Department of Physical Therapy, University of Wisconsin-LaCrosse, LaCrosse, WI Epidemiological studies suggest that prolonged sitting increases all-cause mortality; yet, physiological causes underpinning “sitting disease” remain elusive. PURPOSE: To investigate the acute effects of a moderate intensity exercise bout on cardiometabolic function during prolonged sitting. METHODS: We evaluated cardiometabolic function during prolonged sitting (5 h) in 10 adults with and without 30 min of moderate exercise leading up to the sitting bout. Mean arterial blood pressure (MAP), heart rate (HR), and posterior tibial artery blood velocity (PTABV) were measured at baseline (BL) and every hour subsequently. Blood glucose was measured at BL, 3, and 5-h, with consumption of a caloric beverage at 1 h. Copyright © 2015 by the American College of Sports Medicine. 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