Research Article Molecular Epidemiology of SARS-CoV-2 within Accra Metropolis Postlockdown Frank T. Aboagye , 1,2 Lawrence Annison , 1 Henry Kwadwo Hackman , 1 Maame E. Acquah, 3 Yvonne Ashong , 4 Isaac Owusu-Frimpong, 2 Bill C. Egyam, 5 Sharon Annison, 6 George Osei-Adjei, 1 and Samuel Antwi-Bafour 7 1 Department of Medical Laboratory Technology, Faculty of Applied Sciences, Accra Technical University, Accra, Ghana 2 Biomedical and Public Health Research Unit, Council for Scientifc and Industrial Research–Water Research Institute, Accra, Ghana 3 WestAfricanCentreforCellBiologyofInfectiousPathogens,CollegeofBasicandAppliedSciences,UniversityofGhana,Legon, Accra, Ghana 4 DepartmentofParasitology,NoguchiMemorialInstituteofMedicalResearch,CollegeofMedicalSciences,UniversityofGhana, Legon, Accra, Ghana 5 Department of Molecular Biology, MDS Lancet Laboratories Ghana Limited, East Legon, Accra, Ghana 6 Department of Epidemiology and Disease Control, School of Public Health, University of Ghana, Legon, Accra, Ghana 7 Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Korle-Bu, Accra, Ghana Correspondence should be addressed to Lawrence Annison; larryannison@gmail.com Received 31 October 2023; Revised 31 January 2024; Accepted 11 March 2024; Published 28 March 2024 Academic Editor: Jay Brown Copyright © 2024 Frank T. Aboagye et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Introduction. Currently, sequencing has been the only tool for the identifcation of circulating severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants. However, it is known to be an expensive and laborious approach involving high technical expertise. Considering the reduced adherence to preventive measures postlockdown in Accra, this study presents an alternative method that leverages polymerase chain reaction (PCR) to identify circulating SARS-CoV-2 variants in the Accra Metropolis postlockdown. Methods. Tis prospective cross-sectional study was conducted between July and December 2022. Nasopharyngeal samples were collected from 268 consenting participants. Samples were subjected to nucleic acid extraction and followed by real- time polymerase chain reaction for the detection and quantifcation of SARS-CoV-2 RNA. SARS-CoV-2 positive samples were subsequently subjected to variant identifcation using rapid PCR. Findings. Te prevalence of SARS-CoV-2 within the Accra Metropolis was 30.2%. Te majority of the SARS-CoV-2 infection was diagnosed in females, participants aged 41–50years, and symptomatic participants. Participants aged ≤10 years and females recorded the highest viral load while participants aged 41–50years recorded the highest number of infections. Te SARS-CoV-2 variants detected were Alpha (64.2%), Delta (22.2%), and Omicron (13.6%). Predictors of SARS-CoV-2 infection identifed were chills, cough, headache, body weakness, sore throat, and dyspnoea in order of decreasing association with SARS-CoV-2 infection. Tere was a strong association between symptom status, gender, age, and SARS-CoV-2 infection. Conclusion. Tere was a high prevalence of SARS-CoV-2 within the Accra Metropolis postlockdown within the sampling period. Te Alpha variant of SARS-CoV-2 is the predominant circulating variant, and persons presenting with symptoms are most likely to be diagnosed with COVID-19. Children aged ≤10 years serve as a reservoir for infection transmission. Hindawi Advances in Virology Volume 2024, Article ID 2993144, 12 pages https://doi.org/10.1155/2024/2993144