dental materials 33S (2017) e1–e92 e55 strains were from Escherichia coli ATCC 25922 (Gram negative) and Staphylococcus aureus ATCC 25923 (Gram positive) used the paper disc method and the wells method. The reading (24 h and 48 h) was done by measuring the diameter of the inhibi- tion zone: the larger the inhibition zone diameter, the greater the susceptibility of bacteria to antimicrobial substance. Results: Values for elastic modulus (E) and flexural strength (FS) at 24 h of the 0.8% concentration GO-ZrO 2 presented the higher values, but after 1 month of storage this concentration showed the lower values of all. For the two bacteria tested the composites with graphene loaded presented inhibition of growth for all concentration. Also, by the wells method it may notice a higher antibacterial activity and especially for the GZ2 against E. coli (12 mm) and GS2 against S. aureus (13 mm). The largest diameter of bacterial inhibition zone (14 mm) was obtained by the paper disc method for GS1, GS2 against S. aureus and GZ2 against E. coli. Conclusions: The bacterial inhibition assay revealed that exist an antibacterial activity for all samples, but most good activity is against S. aureus bacteria for composites with GO- SiO 2 graphene. Values flexural strength after 1 month of storage was lower. Acknowledgement: This paper was supported by a grant of MEN-UEFISCDI, Project No. 230/2014 and 101PED/2017. http://dx.doi.org/10.1016/j.dental.2017.08.107 107 NaOCl pretreatment and 3-year retention of cervical composite restorations M. Favetti 1 , T. Schroeder 1 , A.F. Montagner 2 , R.R. Moraes 1,∗ , T. Pereira-Cenci 1 , M.S. Cenci 1 1 Federal University of Pelotas, Brazil 2 Federal University of Santa Maria, Brazil Purpose/aim: This split-mouth, randomized controlled clinical trial evaluated the 3-year clinical performance of composite resin restorations in non-carious cervical lesions (NCCLs) performed with or without a deproteinization pre- treatment with 10% NaOCl solution on etched dentin (experimental). The control group used a placebo solution (water). Materials and methods: In total, 30 patients with 100 cer- vical restorations were included in the study. The NCCLs were evaluated according to lesion shape (“U” or “V”), lesion length and height (in mm), relation to the gingival margin (supragin- gival, gingival level, or subgingival), presence of wear facets, dentin sensitivity, and pulp vitality. Randomization consid- ered the tooth group. A two-step, etch-and-rinse adhesive system and a nanofilled composite were applied according to the manufacturer’s directions. The placebo or NaOCl solu- tions were applied for 60 s after acid etching and rinsed with water. Clinical evaluations were carried out after 1 week, 6, 12, 24, and 36 months using the FDI criteria. Differences between frequencies were assessed by the Exact Fisher test. Survival analysis was performed using the Kaplan–Meier method. The log-rank test was used to evaluate differences between the survival curves. Unadjusted Cox-regression models with shared frailty were used to verify the association between treatment and the risk of failure over time (˛ = 0.05). Results: The average lifetime of the restorations was 2.86 years. At 36 months, the annual failure rate was 9% for the control and 17.8% for the experimental group. Most lesions had a V wedge shape (60%), 48% had a depth below 1 mm, and 49% had a height between 1 and 3 mm. Most NCCLs were present in premolars (58%). In the survival analysis, no sig- nificant differences were observed between the control and experimental groups (p = 0.077) or for the number of teeth in the mouth (p = 0.320), whereas mandibular restorations (p = 0.039) and restorations in premolars (p = 0.013) had signif- icantly lower clinical survival. The experimental group failed 40% more often compared to the control group, but the associ- ation was not significant (p = 0.075). From the sample, 37.5% of patients never had tooth sensitivity, 56.3% had improvement in sensitivity after restoration, and 6.3% reported the restored teeth remained sensitive (all from the control group). Conclusions: In conclusion, the deproteinization technique did not improve the retention of class V restorations in NCCLs or the failure rates during 36 months of clinical follow up. http://dx.doi.org/10.1016/j.dental.2017.08.108 108 Resin composite restoration survival: Preliminary results on systematic review K.M.S. Moreira ∗ , J. Puppin-Rontani, R.M. Puppin-Rontani Piracicaba Dental School-University of Campinas, Brazil Purpose/aim: This study determined the clinical evidence of the most suitable treatment/material for resin composite restoration survival in primary and permanent teeth. Materials and methods: The PRISMA guidelines were fol- lowed. Studies were searched on PubMed, Embase and Scielo databases without language restrictions, from August 1977 to January 2017. The MeSH headings used were “adhesive” and “restoration” and “composite”. The analysis was limited to clinical trials that compared adhesives and/or resin compos- ites related to restoration survival in primary and permanent teeth and had at least 12 months of follow-up. The primary outcome evaluated was retention, followed by recurrent caries and marginal discoloration. After title, abstract and full ver- sions reading, the data were extracted and the studies were subjected to the evaluation of risk of bias according to pre- determined criteria of Cochrane Collaboration’s tool. Narrative synthesis of those included studies was performed. Results: Among the 905 papers identified in the search, 117 fulfilled the inclusion criteria. Fifteen studies were of low risk of bias. No statistical difference was found in the retention of different adhesives and resin composites in 12 studies. However, two studies showed worst performance of one-step self-etch adhesive, for retention, recurrent caries and marginal discoloration, in comparison to two-step self- etch, two and three step etch-and-rinse adhesives. One study showed better results on marginal discoloration when selec- tive etching of the enamel for self-etch adhesives was used.