Journal of Pharmaceutical Negative Results ¦ Volume 13 ¦ Special Issue 5 ¦ 2022 269 Assessment Of Antiarthritic Potential Of Carica Papaya L. Leaves Extract And Fractions: Histopathological Analysis Man Singh 1 *, Danish Ahmed 2 , Himanshu Pandey 3 , Rahul Deo Yadav 4 , Shradhanjali Singh 5 , Shanti Bhushan Mishra 6 * 1,4 Department of Pharmacy, MLN Medical College, Prayagraj 211001, India * 1, 2, 4 Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj 211008, India 3 Centre for Teacher Education, Central Institute of Higher Tibetan Studies, Sarnath, Varanasi, 221007, India 5,6 United Institute of Pharmacy, Prayagraj 211010, India *Corresponding Author: Man Singh, *Department of Pharmacy, MLN Medical College, Prayagraj 211001, India, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj 211008, India, Email: mansinghmpharm@gmail.com DOI: 10.47750/pnr.2022.13.S05.38 Carica papaya L has been traditionally claimed for curing inflammation hence the present work exemplifies the anti-arthritic potential of crude leaves extract and its fractions in arthritic models. Screening on this plant has established presence of kaempferol which might possess antiarthritic properties. Kaempferol was quantitatively estimated by high performance thin layer chromatographic method in chloroform fraction and was found to be 0.08% w/w. Arthritis was induced by intraplantar administration considering formaldehyde (2%v/v), turpentine oil emulsion (10%v/v) and complete Freund’s adjuvant (1%v/v) in left hind paw of rats. Paw oedema, body weight, haematological parameters, level of serum enzyme markers, biochemical parameters, interleukin-6 and histopathological analysis were observed in all groups. Present experimental work justifies that in chronic arthritis, iinvolvement of interleukin-6 is associated with non-articular manifestations considering joint inflammation including anaemia. The plant extract and chloroform fraction possess dose dependent antiarthritic effect in acute and complete Freund’s adjuvant induced arthritic model by ameliorating the level of serum enzyme biomarkers, haematological and biochemical parameters, body weight abnormalities, interleukin-6. The experimental explanation established that extract and its fraction of Carica papaya L. revealed anti-arthritic potential. Keywords: Antiarthritic, Kaempferol, Complete Freund’s adjuvant, Carica papaya, Fraction INTRODUCTION Rheumatoid arthritis (RA) is a symmetric, provocative, peripheral polyarthritis of unidentified etiology. Early detection and management with disease-modifying antirheumatic drugs (DMARDs) is imperative to attain control on disease and deterrence of joint injury and disability [1] . In view of safer medication for RA, fascinatingly peoples were concerned using diversified herbs and other natural products which suggest a vast resource for anti-arthritic agents. Indians used Carica papaya traditionally to treat various diseases. Traditionally leaves of Carica papaya L. are used in severe jaundice [2] , to expel guinea worm [3] , as a poultice 8 [4] , in fracture healing [5] , constipation and indigestion [6] . Scientific exploration of this plant revealed its abortifacient [7] hypoglycaemic [8] , hypolipidemic [9] , gastroprotective [10] , antimicrobial [11] , antimalarial [12] , and wound healing properties [13] . C. papaya leaf has been accounted to include phytochemical compounds suggesting alkaloids, flavonoids, quinines, tannins and steroids which may together add on the way to its biological actions [14, 15] . Adding together to the profusion of phenolic compounds along with antioxidant actions acknowledged in a methanol extract of C. papaya leaf [16] , the alkaloid carpaine, pseudocarpain, alkaloids, dehydrocarpaine I and II, choline, vitamine C and E, carposide were considered to foremost provider on the way to the leaf’s therapeutic properties [17] . The efficacy of C. papaya L leaf resides in its consideration in thrombocytopaenia management through dengue infection. [18] Complete Freund’s Adjuvant induced arthritic animal model resembles human rheumatoid arthritis and hence arthritic manifestations need to be scientifically explored. The present study explored phytoconstituents in CPEE and kaempferol in CPCF along with antiarthritic activity of plant. MATERIAL AND METHODS Plant collection The leaves of Carica papaya L were collected from herbal garden of United institute of Pharmacy, Naini, Prayagraj in the month of March 2019 and air-dried at 40 0 C. The plant was authenticated by taxonomist Dr. Arti Garg from Botanical Survey of India, Prayagraj and voucher specimen has been submitted in departmental herbarium of BSI with Accession No. 104699.