Page 1/27 Ecient expression in the prokaryotic host system, purication and structural analyses of the recombinant human ACE2 catalytic subunit as a hybrid protein with the B subunit of cholera toxin (CTB-ACE2) Maryam Ghahramani Shiraz University Mohammad Bagher Shahsavani Shiraz University Seyed Hossein Khaleghinejad Shiraz University Ali Niazi Shiraz University Ali Akbar Moosavi-Movahedi University of Tehran Reza Youse ( ryouse@shirazu.ac.ir ) Shiraz University Research Article Keywords: Human ACE2 enzyme, Cholera toxin B subunit, Optimizing expression, Purication Posted Date: July 3rd, 2023 DOI: https://doi.org/10.21203/rs.3.rs-3121889/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Additional Declarations: No competing interests reported. Version of Record: A version of this preprint was published at The Protein Journal on November 28th, 2023. See the published version at https://doi.org/10.1007/s10930-023-10164-y.