Version 1.3, 07/01/2020, Page 1/38 Protein delivery in intermittent and continuous enteral nutrition with a protein-rich formula in critically ill patients {1} A protocol for the prospective randomised controlled proof-of- concept Protein Bolus Nutrition (Pro BoNo) study {1} Simona Reinhold 1 *, Désirée Yeginsoy 1 *, Alexa Hollinger 1 , Atanas Todorov 1 , Lionel Tintignac 2 , Michael Sinnreich 3 , Caroline Kiss 4 , Caroline E. Gebhard 1 , Balázs Kovács 5 , Bianca Gysi 1 , Lara Imwinkelried 1 , Martin Siegemund 1 1 Intensive Care Unit, University Hospital Basel, Basel, Switzerland 2 Department of Biomedicine, University Hospital Basel, Basel, Switzerland 3 Departments of Biomedicine and Neurology, Centre of Neuromuscular Diseases, University Hospital Basel, Basel, Switzerland 4 Department of Clinical Nutrition, University Department of Geriatric Medicine Felix Platter, Basel, Switzerland 5 Department of Radiology, Musculoskeletal Diagnostics, University Hospital Basel, Basel, Switzerland * Both authors contributed equally to study design and writing of the manuscript. Sponsor {5b} Martin Siegemund, MD Deputy Head of Intensive Care Unit University Hospital Basel Tel. +41 61 328 64 14 Corresponding author Alexa Hollinger, MD Intensive Care Unit University Hospital Basel Spitalstrasse 21 CH-4031 Basel, Switzerland Tel. +41 61 556 58 95 E-mail: alexa.hollinger@usb.ch