Methods: Fifty-eight participants who were either normal weight (NW) or overweight/obese (OW), and either attempting or not attempting weight loss (WL), exercised at a moderate and vigorous intensity for 25 minutes. Subsequently, they estimated how many calories they expended, and the amount of food equivalent to the calories expended. Results: The OW-noWL group (150166 kcal) significantly over- estimated vigorous exercise expenditure compared to the NW-WL group (-46166 kcal) and OW-WL group (-10180 kcal) (P<0.05). The OW-noWL group (moderate 50108 kcal; vigorous 106+166 kcal) also significantly overestimated how many calories were in a meal compared to both WL groups under both exercise conditions by -55 to -97 kcal (P<0.05). Individuals had a large range in ab- solute error in calorie estimation of exercise and food (0.3-1160 kcal), with mean values not differing between groups (P>0.05). Between exercise conditions, only the OW-noWL group signifi- cantly overestimated energy expenditure during vigorous exercise to a great extent (12583 kcal) compared to moderate exercise (P<0.05). Conclusions: Overweight adults not attempting weight loss may be more likely to overestimate calories during exercise and in a meal compared to adults who are trying to lose weight. However, there is a large variability in the accuracy of calorie estimation for all groups, which may have implications for future weight man- agement success. P8.03 Deprivation by Diet Alone or by Aerobic Exercise Alone: How Modality of an Acute 3 Day Intervention Can Differently Impact Bias for Sweet Taste and Fat Preference JAMEASON D. CAMERON * 1 , GARY S. GOLDFIELD 1 , GRAHAM FINLAYSON 2 , JOHN E. BLUNDELL 2 , ERIC DOUCET 3 1 Children’s Hospital of Eastern Ontario-Research Institute, Ottawa, ON, Canada 2 University of Leeds, Leeds, United Kingdom 3 University of Ottawa, Ottawa, ON, Canada Objectives: It is unclear how modality of energy deprivation can impact food preference. The objectives were to examine how mo- dality of an acute energy deprivation impacts appeal bias for fat and sweet foods. Methods: Ten males aged 23.75.1 yrs. participated in this repeated-measures crossover design. After 3 days of normal eating (CON), subjects were randomized to an acute 3-day -25% daily needs energy deficit induced either by diet only (DER) or by exer- cise only (DEX). ‘Wanting’ and ‘liking’ fat and sweet appeal bias (Leeds Food Preference Questionnaire), tested across a lunch meal once in the fasted state and once in the fed state, was measured after exposure to each condition. Results: For ‘explicit liking’ sweet appeal bias there was a main effect of condition (p¼0.030), with greater sweet appeal bias at DER compared to CON. For ‘explicit wanting’ fat appeal bias there was a condition and time interaction (p¼0.049) with greater values at DER, followed by DEX when fasted (versus CON). Also when fasted there was a condition by time interaction (p¼0.04) for greater ‘implicit wanting’ sweet appeal bias in DER and DEX compared to CON. Conclusions: Obesity interventions targeting 25% energy restric- tion by either diet or exercise need to consider that both forms of energy deprivation acutely increase ‘explicit wanting’ fat bias and ‘implicit wanting’ sweet bias; additionally, greater overall ‘explicit liking’ bias for sweet foods in DER (but not DEX) indicates the need to further study if such preferences lead to a susceptibility to overeating specific macronutrients. P8.04 The Impact of Childcare Attendance on Physical Activity in Young Children SARAH E. CARSLEY * 1, 2 , LISA Y. LIANG 1 , PATRICIA C. PARKIN 2 , JONATHON MAGUIRE 1 , CATHERINE S. BIRKEN 1, 2 1 University of Toronto, Toronto, ON, Canada 2 The Hospital for Sick Children, Toronto, ON, Canada Background: Physical activity (PA) is important for healthy growth and development in early childhood. Recent studies have shown that the majority of time spent in childcare is sedentary. Objective: To determine if there is an association between child- care attendance, specifically daycare or preschool, and outdoor free play of young children outside of a childcare setting. Methods: Healthy children ages 1 to 5 years old recruited to The Applied Research Group for Kids! (TARGet Kids!), a community- based primary care research network, from January 2009 to August 2011 were included in this study. Parent reported childcare use and PA as well as potential confounding variables were collected through questionnaires. Multivariable linear regressions, stratified by age group, were used to determine the association between childcare attendance and physical activity, adjusted for potential confounders; age, sex, maternal ethnicity, maternal education, neighbourhood income, and season. Results: There were 2810 children included in this study. Children aged 1 to 2 years old who attended daycare had 14.7 minutes less of outdoor free play compared to children who did not attend daycare (p<0.01). This result was also seen in children 3 to 5 years old who had 9.64 minutes less PA per day (p<0.01) if they attended daycare. This association remained significant after categorizing daycare into part-time, full-time, and no daycare. Discussion: Children who spend more time in childcare have less outdoor free play at home. Parents may be relying on childcare to provide opportunity for physical activity and may not be encour- aging outdoor play at home. P8.05 How Independent Mobility Influences Outdoor Active Play among 8-11 Year Old Children THOMAS FERRAO Queen’s University, Kingston, ON, Canada Objective: Active play refers to unstructured and self-directed physical activity performed in children’s free time. Active play can contribute to greater physical activity levels and subse- quently help prevent obesity. The extent to which parents in- fluence the active play domain of physical activity is not well established. One way in which parents could negatively influence their child’s active play is by limiting their independent mobility, or the extent to which they can travel around their neighbour- hood without adult supervision. The objective of this study is to determine if independent mobility is associated with active play among 8-11 year olds. Methods: Participants consist of dyads of 8-11 year old children and one of their parents/guardians. Participants are from across Canada and the United States. To measure active play, parents completed a web-based survey consisting of questions measuring how frequently their child engages in outdoor play in various lo- cations. Independent mobility was measured by asking parents how far their child is allowed to roam from home alone and with friends without adult accompaniment. All survey questions were taken or adapted from existing validated instruments. Generalized linear models will be used to examine the relationship between the independent mobility and active play frequency of children, while controlling for several covariates. Abstracts / Can J Diabetes 39 (2015) S38eS74 S64