Molecular Detection Of Staphylococcus Aureus … Mai A. S., Boghdady et., al. Kafr El-Sheikh Vet.Med.J. Vol. 1 No.1 (2003) 23 Kafrelsheikh Vet. Med. J. Vol. 16 No. 2 (2018) (23-43) MOLECULAR DETECTION OF STAPHYLOCOCCUS AUREUS ENTEROTOXINS ISOLATED FROM MASTITIC SHE-CAMELS Mai A.S.,Boghdady 1 , Alsagher .O.A. Ali 2 , , Hassan. Y.A. H.Mahmoud 2 , Khaled Ibrahim El-Ekhnawy 3 , Adel E.A. Mohamed 2* 1 Animal Health Researches Institute, Aswan branch, Egypt. 2 Department of Animal Medicine, faculty of Veterinary Medicine, South Valley University, Qena city, Qena 83523, Egypt. 3 Animal Health Researches Institute, Dokki, Giza, Egypt. * Corresponding author's e-mail: adel.mohamed@vet.svu.edu.eg ABSTRACT A total of 150 raw milk samples were collected from she camels from Aswan, 100 from individual breed in Daraw and 50 from pastoral camels in Shalateen in Egypt. First, clinical examination revealed no cases with clinical mastitis. Second, the collected milk samples were tested by California Mastitis Test and the results revealed that the percent of subclinical mastitis was (39.3%) for total number of milk samples, but the ratio in Daraw (48%) higher than in Shalateen (22%). Third, by conventional culture method, 20 % of samples were positive to Staphylococcus spp. Fourth, Staphylococcal isolates were identified by conventional biochemical test, (46.7%) of these isolate showed positive for coagulase test. Fifth, these coagulase positive isolates submitted for molecular identification targeting 16S rRNA gene (Staphylococcus genus specific), nuc gene (S. aureus species specific) and Staphylococcal enterotoxins genes (SEA, SEB and SEC) by multiplex PCR, (92.9%) positive for 16s rRNA and confirmed as