Abstracts / European Journal of Obstetrics & Gynecology and Reproductive Biology 234 (2019) e1–e131 e21 Gynaecological Oncology Session 06 – Gynaecological ultrasound and ovarian cancer Reference: A2113MH Discrimination between benign and malignant pelvic masses using the risk malignancy index in the Maltese scenario Marlon Harmsworth ∗ , Mark Cordina, John Thake Mater Dei Hospital, Msida, Malta Introduction: Ovarian cancer is the commonest cause of cancer death from gynaecologic tumours in the developed world, with one of the most common presentations being a pelvic mass. Increasing the ability to ascertain in advance whether an ovarian neoplasm is benign or malignant in the pre-operative assessment will aid in appropriate referral and in planning the optimal surgery. CA125, Ultrasound Score and Risk of Malignancy Index (RMI) are known methods that can be used to triage such masses. Nonetheless, no data exists as to the performance of such indices in the Maltese local scenario. Objective: The aim of this study was to evaluate the ability of CA125, Ultrasound Score and Risk Malignancy Index to differentiate a benign from a malignant pelvic mass in the Maltese local scenario. Methods: This is a retrospective study of 97 women admit- ted to the Department of Obstetrics and Gynaecology at Mater Dei Hospital, between September 2015 and February 2016 for surgical investigation of pelvic masses. To diagnose ovarian cancer the sen- sitivity, specificity, and positive predictive value of serum CA125, ultrasound findings and menopausal status were calculated first individually and then as combined together into four risk malig- nancy indices, RMI 1–4. Discussion and conclusions: The receiver operating charac- teristic (ROC) curves demonstrated that all four risk malignancy indices were more accurate than menopausal status, serum CA125 levels and ultrasound score separately. We found that there was no statistically significant difference in the performance of the four different RMIs in discriminating malignancy. We concluded that all of the four risk malignancy indices described can be utilised for the selection of cases for optimal treatment within the Maltese clini- cal scenario. These methods are simple techniques that can be used even in less-specialised gynaecology clinics to facilitate the triaging of cases for referral to an oncology tertiary unit. https://doi.org/10.1016/j.ejogrb.2018.08.196 Gynaecological Oncology Session 06 – Gynaecological ultrasound and ovarian cancer Reference: A2242R Transvaginal sonography in endometrial carcinoma: Preoperative assessment of deep myometrial invasion and its impact on surgical planning Rok ˇ Sumak 1,∗ , Maja Pakiˇ z 1,2 1 Clinic of Gynaecology and Perinatology, University Medical Centre Maribor, Maribor, Slovenia 2 Faculty of Medicine, University of Maribor, Maribor, Slovenia Introduction: Accuracy of ultrasonographic preoperative assessment of deep miometrial invasion (MI) is crucial in surgi- cal planning of patients with apparent FIGO stage I endometrial carcinoma (EC), since according to latest ESMO-ESTRO-ESGO guide- lines for patients with FIGO stage IA, G1/2 endometrioid carcinoma systematic lymphadencetomy (LND) is no longer recommended. Objective: To evaluate the diagnostic accuracy of transvaginal sonography (TVS) in preoperative assessment of MI at our series and its relation to surgical over/undertreatment of endometrial cancer patients. Methods: In the retrospective study we included all consecu- tive 104 patients with apparent FIGO stage I endometrioid G1/2 EC patients who were treated at our department in years 2015–2017. All patients underwent preoperative TVS. When feasible sentinel node biopsy (SNB) was performed instead of systematic LND to evaluate retroperitoneal lymph nodes. The accuracy of preoper- ative TVS was estimated according to the final histopathological report. Discussion and conclusions: MI was assessed in 81.7% (85/104) of patients, in others the US poor quality image was the limitation for MI estimation. Sensitivity, specificity, PPV and NPV were 82.9%, 69.8%, 64.2%, 86.3%, respectively. 14 out of 19 patients with false positive deep MI had LND performed unnecessarily. 7 patients had false negative estimation of deep MI (6.7%); since SNB was per- formed, only 2 of them needed second surgery for staging purpose. Diagnostic accuracy of preoperative TVS for estimation of deep MI is moderate. Around every fifth patient had false positive estima- tion of deep MI, hence LND would be unnecessarily performed. There were only 6.7% false negative preoperative estimations of deep MI. Those patients would benefit from retroperitoneal lymph node assessment. According to our experience SNB might be the solution especially when the ultrasound image quality is poor or when there are no preoperative signs of deep MI to minimize the need for second operation. https://doi.org/10.1016/j.ejogrb.2018.08.197 Gynaecological Oncology Session 06 – Gynaecological ultrasound and ovarian cancer Reference: A2072AK Phagocytic function of peripheral monocytes and neutrophils in ovarian cancer Anna Rebeka Kovács 1,∗ , Róbert Póka 1 , Sándor Szücs 2 , László Pál 2 , Rudolf Lampé 1 1 University of Debrecen, Faculty of Medicine, Department of Obstetrics and Gynecology, Debrecen, Hungary 2 University of Debrecen, Faculty of Public Health, Department of Preventive Medicine, Debrecen, Hungary Introduction: Neutrophil granulocytes and monocytes are phagocytes of the innate immune system and play an important role in the defense against tumor cells. Recently, several studies have been reported describing changes in the functions of tumor- infiltrating immune cells in various types of cancer (e.g. ovarian cancer). However, fewer studies address the role of peripheral immune cells in the pathogenesis of cancer. Objective: Aim of our study was to determine the phagocytic function of peripheral monocytes and neutrophil granulocytes from epithelial ovarian cancer (EOC) patients in comparison with cells from healthy controls. Methods: We investigated peripheral blood samples from 12 patients with advanced stage (IIIC) of EOC and 14 healthy controls. From blood samples we isolated the monocytes and granulocytes by using Ficoll density gradient. Separated monocytes and neu-