How to Cite: Debata, J., Kumar, V. R., Mohanty, D., Mohanty, D., Nirosha, B., Panda, O. P., Kumar, C. P., & Bakshi, V. (2022). Development, in-vitro characterization and ex-vivo permeation study of metronidazole loaded mucoadhesive ethosomal gel for the local treatment of oral cavity infection. International Journal of Health Sciences, 6(S5), 6059–6070. https://doi.org/10.53730/ijhs.v6nS5.10036 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 27 Feb 2022, Manuscript revised: 9 April 2022, Accepted for publication: 18 June 2022 6059 Development, in-vitro characterization and ex- vivo permeation study of metronidazole loaded mucoadhesive ethosomal gel for the local treatment of oral cavity infection Jitendra Debata Associate Professor, Guru Nanak Institutions Technical Campus, School of Pharmacy, Ibrahimpatnam Hyderabad, Telangana, Pin:501506 V. Ravi Kumar Associate Professor, Guru Nanak Institutions Technical Campus, School of Pharmacy, Ibrahimpatnam Hyderabad, Telangana, Pin:501506 Dibyalochan Mohanty Associate Professor, Department of Pharmaceutics, School of Pharmacy, Anurag University, Hyderabad, Telangana, Venkatapur, Pin:500088 B. Nirosha Assistant Professor, Department of Pharmaceutics, School of Pharmacy, Anurag University, Hyderabad, Telangana, Venkatapur, Pin:500088 Om Prakash Panda Assistant Professor, Centurion University of Technology and Management, Odisha, India Ch Praveen Kumar Associate Professor, Swathi College of Pharmacy, Nellore, Andhra Pradesh,Pin:524320 Vasudha Bakshi Professor & Dean, School of Pharmacy, Anurag University, Hyderabad, Telangana, Venkatapur, Pin:500088 Abstract---The present study deals with the formulation, characterization of Metronidazole loaded ethosomal gel for treating oral infection. The ethosomal system of Metronidazole comprises of 2- 5% phospholipids, 20-50% ethanol, 10% of propylene glycol, 10% of cholesterol and 10% v/v aqueous phase. Cold method was applied to develop ethosome. The prepared Metronidazole loaded ethosome was evaluated for vesicle size, entrapment efficiency,The Optimized