_____________________________________________________________________________________________________ # Lecturer; ≡ Professor; ⱷ Associate professor; *Corresponding author: E-mail: vishnupriya@saveetha.com; Journal of Pharmaceutical Research International 33(61A): 177-185, 2021; Article no.JPRI.82136 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Glyphosate Caused Detrimental Changes in Enzymatic Antioxidants in Rats R. Prenetha a , S. Kavitha b# , V. Vishnupriya b*≡ , J. Selvaraj bⱷ and R. Gayathri bⱷ a Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences Saveetha University, Chennai-600 077, India. b Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600 077, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i61A35453 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/82136 Received 20 October 2021 Accepted 27 December 2021 Published 28 December 2021 ABSTRACT Background: Glyphosate is the most enormously used broad spectrum herbicide in the world. Current assessment of carcinogenic capability of glyphosate-based herbicides by various regional, national, and international agencies have endangered the controversy. Antioxidant enzymes are often used as biomarkers of oxidative stress. Among the biomarkers superoxide dismutase, catalase and glutathione peroxidase were essential in conservation of homeostasis of cell to function as normal being. Aim: To investigate glyphosate induced detrimental changes in the enzymatic antioxidants in experimental rats. Materials and Methods: Adult male wistar albino rats were divided into 4 groups, each consisting of 6 animals. Group 1 consists of Normal control rats, Group 2 consists of Glyphosate treated at a dose of 50mg/kg body weight/day. Group 3 consists of Glyphosate treated at a dose of 100 mg/kg body weight/day. Group 4 consists of Glyphosate treated at a dose of 250 mg/kg body weight/day. The experimental period was 16 weeks. All chemicals and reagents used in this study were purchased from sigma chemical company, USA. Adult male albino rats weighing 180-200g were used for the study. Parameters analyzed were assay of Superoxide Dismutase Catalase, Glutathione Peroxidase. The data were analyzed statistically by one-way analysis of variance Original Research Article