J. Nanoanalysis., 5(3): 182-187 Summer 2018 RESEARCH ARTICLE Histopathological efects of zinc oxide nanopartcles (ZnO) on skin and muscle tssues of rats Parisa Saberi-Hasanabadi, Sepideh Arbabi-Bidgoli Department of Toxicology, Faculty of Pharmacy, Islamic Azad University-Pharmaceutcal Sciences Branch, (IAUPS), Tehran, Iran Received: 2018-04-16 Accepted: 2018-07-13 Published: 2018-08-10 ABSTRACT Although ZnO nanopartcles possess novel propertes that make them available to a wide range of applicatons, the questons regarding their safety may arise when they come in direct contact with biological systems (such as skin, lungs, and tssues). In this study, we evaluated the possible toxic efects of diferent dosages of zinc oxide nanopartcles (25, 50 and 100 mg/kg) in three treatment groups in four weeks on skin and muscle tssues of treated rats. For toxicological assessments, male rats weighing 150 to 200 g were exposed to three diferent concentratons of zinc oxide nanopartcles (25, 50 and 100mg/kg) in an acute study. Toxic responses were assessed by clinical and histopathologic parameters. In all experimental animals the sites of exposure were scored for any type of dermal toxicity and compared with the negatve control group. All changes were compared with the negatve control group and the results were analyzed by one-way analysis of variance (ANOVA). Results have been indicated that the mean levels of the histopatological injuries were scored in experimental groups and showed no signifcant diference with control group that mean,the number of vacuole degeneraton showed signifcant increase in high dose group (p<0.05). The results showed that the topical applicaton of zinc oxide cannot make remarkable efects on the skin and skeletal muscle tssue of the rats in low and medium doses. Although, we did not fnd any harmful efects on the use of low and medium dosages of zinc oxide nanopartcles on the skin and musculoskeletal system, we cannot ignore the observatons regarding the sensitvity of cells and tssues to the potental cytotoxic efects this kind of nanopartcles. Therefore, it is suggested to conduct further researches on the complex toxicity mechanism of zinc oxide nanopartcles in living organisms. Keywords: Rats, Skin and Tissues Histopathology, Toxicity, Zinc Oxide Nanopartcles © 2018 Published by Journal of Nanoanalysis. How to cite this artcle Saberi-Hasanabadi P, Arbabi-Bidgoli S. Histopathological efects of zinc oxide nanopartcles (ZnO) on skin and muscle tssues of rats. J. Nanoanalysis., 2018; 5(3): 182-187. DOI: 10.22034/jna.2018.542767 ORIGINAL RESEARCH PAPER Tis work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. * Corresponding Author Email: sepideharbabi@yahoo.com INTRODUCTION Nanotechnology has become the forefront of research in the past decade. With the advent of this emerging feld, wide varieties of nanoparticles with exciting characteristics are manufactured and used for a broad range of applications especially in health, medicine and food branches [1]. Because of the special properties including small size and high specifc surface area, the biological safety of the nonmaterial has received wide attention. Zinc oxide nanoparticles have been used in a variety of products including semiconductors, catalysts, and paints. Tese particles are increasingly found in consumer products, such as sunscreen, because of their strong UV absorption especially in case of ZnO [8, 11]. Products containing ZnO nanoparticles may release Zn ions, which may be translocated from the environment into the human