Available online at www.sciencedirect.com ScienceDirect Materials Today: Proceedings 19 (2019) 1145–1150 www.materialstoday.com/proceedings * Corresponding author. Tel.: +6012-7237295 E-mail address: norhayatim@uthm.edu.my 2214-7853 © 2019 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the International Conference on Chemical Sciences and Engineering: Advance and New Materials, ICCSE 2018. ICCSE 2018 The Evaluation of Antiurolithiathic Properties of Ananas nanus, Aquilaria malaccensis, Curcuma xanthorrhiza, Pandanus atrocarpus and Garcinia mangostana Nur Halimah Sairi 1 , Norhayati Muhammad 1,2 * , Aslia Natasha Pauzi 1 , Mohd Fadzelly Abu Bakar 1,2 , Balkis Hj. A. Talip 1, 2 , Norazlin Abdullah 1, 2 , Laily B. Din 3 and Nazlina Ibrahim 4 1 Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia (UTHM), Hab Pendidikan Tinggi Pagoh, KM 1, Jalan Panchor, 84600 Muar, Johor, Malaysia 2 Centre of Research for Sustainable Uses of Natural Resources (CoR-SUNR), Universiti Tun Hussein Onn Malaysia (UTHM), Parit Raja, 86400 Batu Pahat, Johor, Malaysia 3 School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia 4 School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia Abstract Medicinal plants was commonly used as an alternatives for treating many ailments including kidney stones disease. These plants provides various promising features as to confered health benefits, lessen the side effect and safe to be consumed. In this regards, a present study was conducted on five Malaysian traditional plant which are A. nanus, A. malaccensis, C. xanthorrhiza, P. atrocarpus and G. mangostana to investigate their inhibition effect towards stone crystallization using in-vitro technique. The plant extracts was obtained by using water decoction method. The turbidity of plant extract and calcium oxalate (CaOx) mixture was observed for inhibition activity in nucleation assay using UV-Vis spectrophotometer. Standard drugs Cystone, was used as positive control in comparison. Among all, the best inhibition rate was possessed by A. Malaccensis extract (77.27%) with no significant different (p<0.05) in comparison to Cystone while the lowest inhibition rate was occupied by C. xanthorrhiza extract (32.95%). The abundance amount of phytochemicals particularly steroid and saponin in A. Malaccensis extract might contributes to the high inhibition activities of calcium oxalate crystallization while low amount of phytochemicals was observed in C. Xanthorrhiza extract. Therefore, it can be concluded that these five traditional plant exhibited antiurolithiatic properties by inhibiting the crystallization of calcium oxalate. © 2019 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the International Conference on Chemical Sciences and Engineering: Advance and New Materials, ICCSE 2018. Keywords: Antiurolithiathic; inhibition; A. malaccensis; A. nanus; C. xanthorrhiza; P. atrocarpus; G. mangostana.