Abstracts from the 41st Annual Meeting / Journal of Dermatological Science 86 (2017) e1–e95 e93 and fibronectin type III domains. TNC is localized in the extracellu- lar matrix and plays a role as a ligand of several types of integrin. TNC also increases mRNA and protein levels of type I collagen in hepatic stellate cells and foreskin fibroblasts. We confirmed that expression of the TnC gene is down-regulated in aged mouse dor- sal skin and aged human buttock skin at RNA and protein levels by RT-PCR, immunohistochemistry, and immunofluorescence analy- ses. In this regards, we propose that down-regulation of the TnC gene is an important characteristic of intrinsically aged skin in mice and humans. http://dx.doi.org/10.1016/j.jdermsci.2017.02.271 L-14[O1-53] Leucine-rich alpha-2-glycoprotein 1 modulates expression of matrix metalloproteinase-1 and type 1 collagen in human dermal fibroblasts So Yun Ahn 1,∗ , Dong Hun Lee 2 , Jin Ho Chung 2 , Seung-Taek Lee 1 1 Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea 2 Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea Differentially expressed genes of RNA sequencing database from dorsal skin tissues of young and old mice were analyzed to iden- tify intrinsic factors of skin aging. We found that expression of Lrg1 gene encoding leucine-rich alpha-2-glycoprotein 1 (LRG1) was significantly downregulated in skin tissues of the old group. Although LRG1 is known as a positive regulator of transforming growth factor-beta signaling pathway, its function and detailed mechanism in skin aging has not been studied well. Our results showed that Lrg1 mRNA level using RT-PCR and LRG1 protein level by western blot and immunohistochemistry markedly decreased in old group compared with young group. His-tagged recombinant human LRG1 (rhLRG1) was expressed and purified from HEK 293 cells. Treatment of rhLRG1 increased secretion of type I collagen and decreased secretion of matrix metalloproteinase (MMP)-1 in human dermal fibroblasts. Moreover, LRG1-induced type I collagen upregulation and MMP-1 down-regulation involves activation of TGF-beta signaling pathway. Taken together, our findings indicate that downregulation of LRG1 promotes skin aging due to increased degradation and decreased synthesis of extracellular matrix com- ponents such as type I collagen. http://dx.doi.org/10.1016/j.jdermsci.2017.02.272 L-15[O3-53] Evaluation of 14 Thai herbs with free radical scavenging activity and enzyme inhibitory behavior Moragot Chatatikun 1,∗ , Anchalee Chiabchalard 2 1 Ph.D. Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand 2 Center for Excellence in Omics-Nano Medical Technology Development Project, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand Overexposure to sunlight induces overexpression of reactive oxygen species (ROS) is problematic resulting in skin aging, hyper- pigmentation and cancer. Development of novel whitening and anti-wrinkle compounds from natural products to address pre- mature aging is important. The purpose of this study was to find products that reduce the reactively of ROS. To determine the anti-aging effects of antioxidant activity, anti-tyrosinase activity and anti-collagenase activity we compared extracts of 14 Thai plant using different solvents (petroleum ether, dichloromethane and ethanol). Scavenging activity ranged from 7.11 ± 0.59% SC to 96.17 ± 0.05% SC by DPPH assay and 8.03 ± 0.54% SC to 99.84 ± 0.07% SC by ABTS assay. The ethanol leaf extract of Ardisia elliptica (Thunb) had the highest antioxidant activity and collagenase inhibition. Interestingly, three plants extracts, which were ethanol fraction of Annonasquamosa L., Ardisiaelliptica Thunb. and Senna alata (L.), Roxb, with high antioxidant activity signifi- cantly inhibited both tyrosinase and collagenase. Our finding, the ethanol fractions of Annona squamosa L., Ardisia elliptica Thunb. and Senna alata (L.) had the highest anti-oxidant activity, suggests that this compound is a promising ingredient in cosmetic products as an anti-wrinkle agents as well as useful in skin whitening creams and lotions. http://dx.doi.org/10.1016/j.jdermsci.2017.02.273 L-16[O1-54] Basal cell carcinoma response to imiquimod is associated with the ratio of Gli1/Gli3 expression Yukihiko Kato 1,2,∗ , Ayano Kanzaki 1,3 , Yoshihiro Umebayashi 2 , Ryoji Tsuboi 3 1 Department of Dermatology, Tokyo Metropolitan Tama Medical Center, Japan 2 Department of Dermatology, Tokyo Medical University Hachioji Medical Center, Japan 3 Department of Dermatology, Tokyo Medical University, Japan Introduction: Imiquimod is used to treat a superficial type of basal cell carcinoma (BCC) in the USA. The anti-tumor effect of this toll-like receptor (TLR) 7 agonist is thought to occur via stimulation of TLR, which activates a Th1 immune response. Wolf reported that imiquimod directly repress hedgehog (HH) signaling by negatively modulating GLI activity in a murine model. Here we discussed 4 cases of BCC treated with imiquimod, and examined the relation- ship between the BCC response to the drug and the expression of the Gli1-related genes. Materials and methods: The subjects were 4 adults, a male and a female in their fifties, and a male and a female in their eighties. Imiquimod cream was applied 3-5 times weekly to their lesion, which was then examined histopathologically. The expression of