ISSN: 2277- 7695 CODEN Code: PIHNBQ ZDB-Number: 2663038-2 IC Journal No: 7725 Vol. 3 No. 1. 2014 Online Available at www.thepharmajournal.com THE PHARMA INNOVATION - JOURNAL Vol. 3 No. 1. 2014 www.thepharmajournal.com Page | 7 Phytochemical research of Galium aparine L. Lipophilic complex and study of its antibacterial activity Olga V. Goryacha 1 , Tetyana V. Ilyina 1 , Alla M. Kovalyova 1 , Nataliya V. Kashpur 2 1. The National University of Pharmacy, Kharkov, Ukraine 2. I.I. Mechnikov Institute of Microbiology and Immunology of Academy of Medical Sciences of Ukraine, Kharkov, Ukraine [Email: Helga_gnosy@mail.ru] From Galium aparine L. herb lipophilic complex of biologically active substances had been obtained for the first time. By means of TLC and chromatography-mass-spectrometry the composition of lipophilic complex had been studied. 3 Sesquiterpenoids, squalene, 7 aromatic compounds, higher alkanes and their derivatives, fatty acids have been identified and quantified; presence of chlorophylls, carotenoids and 2 iridoids had been established. Antibacterial and antifungal activity of Galium aparine herb lipophilic complex and degree of these activities have been studied for the first time. Test-strains of S. aureus, P. aeruginosa and C. albicans were highly sensitive and B. subtilis is moderately sensitive to lipophilic complex of Galium aparine herb. Keyword: Galium aparine L., chromatography-mass-spectrometry, antibacterial and antifungal activity. 1. Introduction Cleavers or (Galium aparine L.) of Madder family (Rubiaceae Juss.) – is one of the most widely spread species of Galium L. genus in moderate climate zone. Previous studies of Cleavers' biologically active substances (BAS) showed presence of hydroxycinnamic acids, coumarins, derivatives of flavones and flavonols in areal part and anthracene derivatives of alizarin type in roots and rhizomes [1, 3, 5] . Our recent research had shown presence of monoterpenoids, sesquiterpenoids, tri- and norterpenoids, aromatic compounds in Galium aparine herb essential oil. Presence of lipophilic compounds with potent antibacterial and antifungal activities was a background for obtaining of complexes of BAS with presumptive antibiotic properties. The object of our research was lipophilic complex of Galium aparine herb. The aim of our research was to study chemical composition, antibacterial and antifungal activity of Galium aparine herb lipophilic complex. 2. Materials and Methods 2.1. Plant material. Galium aparine L. herb was collected in flowering stage in Lugansk region, Ukraine in 2012. The plant identification was performed using voucher specimens of Herbarium of Pharmacognosy Department, the National University of Pharmacy. Lipophilic complex was obtained by the method of exhaustive circulative extraction in Soxhlet apparatus using chloroform as extracting agent. The ratio plant material: extragent was 1: 10, an extraction time – 30 hours. After the end of extraction process the extragent was evaporated at a temperature of 35-40 0 С under vacuum in a vacuum circulation apparatus till complete removal of extragent.