International Jo http:/ Molecular genetic evalua Elsayd E. Hafez 1 , Effat Ba *Corresponding author: Elsayd E. Hafez 1 Department of Plant Protection and Biomolecular Diagnosis, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, Alexandria, Egypt. 2 Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. A M ca in an co al we di te th po Ke Introduction Colorectal cancer (CRC) is a malignant tumor aris wall of the large intestine (the colon). Colorecta the third most common cancer in both men and w second leading cause of cancer-related mortality countries due to the limited prevention and treatm Egypt, due to the changes of eating habits and disease incidence of CRC is increasing by 10 cancer diseases in Egypt (Oncology Center Egypt, 2011). Therefore, the research and deve preventive and anti-cancer agents against CRC 1,2- Dimethyl hydrazine (DMH) is highly specific carcinogens that induce the initiation and pro colorectal carcinogenesis yielding colorectal tum dose-dependent manner in rats, mice and Fluorouracil (5-FU) is a drug that is a pyrimidine used in the treatment of cancer. It is a suicide in through irreversible inhibition of thymidylate synth the family of drugs called the antimetabolites. 5-F systemically for anal, breast, colorectal, oesophageal, stomach skin cancers [3]. About 25% of prescribed dru originate from plants and over 3000 species of reported to have anticancer properties [4]. Cicho very valuable nutritionally, with a high content potassium and vitamin C. The natural components also have many biological activities DOI:10.5138/09750185.1916 This article is distributed under the terms o and redistribution provided that the origina ournal of Phytomedicine 8 (2016) 55 //www.arjournals.org/index.php/ijpm/index Original Research Article ation of Cichorium endivia L. as an against colorectal cancer adr 2 , Yasser Mabrouk 2 , Mohammed El-Seehy 2 , Sarah Abstract Medicinal plants confer protective effects against a wide rang ancer. Cichorium endivia L. has many biological activities a nflammation and hepatoprotective effects. This activity is mai ntioxidant compounds. The effect of cichorium extract and olorectal cancer (CRC) induced by 1, 2- Dimethyl hydrazine ( lbino mice. Where, different doses of cichorium extract (200, 400 ere investigated to determine the significant effective dose ag ifferential display-PCR (DD-PCR), DNA sequencing, histo echniques. The results showed that the cichorium extract dose he best one compared to the others (400 and 600 mg/kg body w owerful therapeutic against colorectal cancer. eywords: Colorectal cancer - DMH - 5-FU - Cichorium endivia L sing from the inner al cancer (CRC) is women and it is the y in the developing ment options [1]. In d frequent sit, the 0-12% of the total Report, Gharbia, elopment of potent C are very urgent. indirect colorectal omotion steps of mour lesions in a d hamsters [2]. e analog which is nhibitor and works hase. It belongs to FU has been given h, pancreatic and ugs in the world plants have been horium endivia L. is t of dietary fibre, s of this vegetable and functions, such as anti-infla effects. This activity is mainly due compounds [5]. The aim of this st endivia L. as an anticancer agent especially on colorectal cancer (CR is to investigate the therapeutic against DMH-induced colorectal ca differential display-PCR (DD-PCR addition, histopathological exami analysis of chromosomal abnorma were investigated. Materials and Methods Cichorium endivia L. collectio Cichorium endivia L. plants were o farm of the Crop Department, Fa University, Egypt. The plant specie Department of Botany, Faculty of Egypt. Plants were washed, dried a [6]. One hundred gram of the leav litter of double distilled water for 72 rpm/30 C). The sample filtrate w evaporator at 40 C, dried using stored until use. of the Creative Commons Attribution License, which p al author and source are credited. 51-557 anticancer agent ah Aggag 2 ge of cancers including colon and functions, such as anti- inly due to its high levels of d fluorouracil (5-FU) against (DMH) was examined in male 0 and 600 mg/kg body weight) gainst colorectal cancer using opathological and cytological e 200 mg/kg body weight was weight). This finding could be a L. - DD-PCR ammation and hepatoprotective e to its high levels of antioxidant tudy is to investigate Cichorium and an immune response drug RC). The aim of the current study effect of Cichorium endivia L. ancer in male albino mice using R) and DNA sequencing. In ination of colon tissues and alities in mice bone-marrow cells on and preparation obtained from the crop research aculty of Agriculture, Alexandria es were correctly identified by the Science, Alexandria University, and then ground using a blender ves powder was soaked in one 2 hours in shaking incubator (150 was concentrated using rotary lyophilizer, weighed and then ISSN: 0975-0185 permits unrestricted use